大孔树脂纯化五味子总木脂素、总三萜工艺研究  

Study on purification of total lignans and total triterpenes from Schisandra Chinensis(Turcz.) Baill by AB-8 macroporous resin

作　　者：莫永俊[1,2] 汪春泉[2] 王琦[1] 赵晓宏[2] 

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118 [2]北京协和医学院中国医学科学院药用植物研究所,北京100193

出　　处：《中国医药导报》2013年第5期106-108,111,共4页China Medical Herald

摘　　要：目的考察大孔树脂纯化五味子总木脂素总三萜的最佳工艺。方法比较了5种不同类型大孔树脂对五味子总木脂素、总三萜、五味子醇甲的吸附解析性能,确定适宜的树脂类型和最佳纯化工艺条件。结果 AB-8型树脂吸附解析效果最好;最佳工艺条件:上样液浓度1.5 mg/mL,最大上样量7BV,蒸馏水洗脱体积9BV,洗脱溶剂为30%乙醇(9BV)+80%乙醇(10BV);纯化后终产品中总木脂素、总三萜、五味子醇甲的质量分数分别为39.55%、13.96%、8.95%。结论本文所报道的梯度洗脱法可显著提高终产品中总木脂素、总三萜、五味子醇甲的质量分数,验证了其工业生产的可能。Objective To establish a technical process for the purification of total lignans, total triterpenes from Schisandra Chinensis （Turcz.） Baill. Methods The adsorption and desorption rate of five types of macroporous resins was calculated to determine the best resin. The dynamic experiments of adsorption and desorption were carried to obtain the optimal parameters. Results The adsorption and isolation effect of AB-8 type resin was the best. The optimal con- ditions were that： the density of sample solution was 1.5 mg/mL, the maximum loading amount was 7 BV as well as distilled water was taken 9BV, solvent of elution was 30% of alcohol （9BV） ＋ 80% of alcohol （10BV）. The content of total lignans, total triterpenes, Schisandrin in the final production could reach 39.55%, 13.96%, 8.95% respectively. Conclusion The method of gradient elution is a better way to purify total lignans and triterpenes. It is possible that this method be used in the industrial production.

关 键 词：五味子 总木脂素 总三萜 五味子醇甲 大孔吸附树脂 

分 类 号：R284[医药卫生—中药学]