低温和获能对猪精子细胞骨架微管蛋白和肌动蛋白表达量的影响  

Effects of capacitation and low temperature on expression of tubulin and actin in boar sperm

作　　者：吴俊波[1] 杨璐茜[1] 曹立朋[1] 田宇[1] 杜宁宁[1] 金一[1] 

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《西北农林科技大学学报（自然科学版）》2013年第1期9-12,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：吉林省自然科学基金项目(201115229)

摘　　要：【目的】探究获能和低温冷冻对猪精子细胞骨架中微管蛋白和肌动蛋白表达量的影响。【方法】利用SDS-PAGE、Western blot技术,分析获能和低温冷冻(-80和-196℃)后猪精子总蛋白质种类以及猪精子细胞骨架微管蛋白和肌动蛋白表达量的变化。【结果】低温处理后,猪精子总蛋白质的种类与新鲜精子相比发生变化,而获能处理后几乎没有差异。低温冷冻导致猪精子细胞骨架微管蛋白和肌动蛋白的表达量较新鲜精子下降,且-196℃冷冻组肌动蛋白的表达量要略高于-80℃冷冻组,但二者微管蛋白的表达量基本没有差异。获能处理后猪精子肌动蛋白表达量略有下降,而微管蛋白表达量基本没有变化。【结论】低温冷冻处理对猪精子总蛋白质的种类以及猪精子细胞骨架微管蛋白和肌动蛋白的表达量有明显影响,而获能处理则影响较小。[Objective] This study aimed to examine the effects of capacitation and low temperature on expression of tubulin and actin in boar sperm. [Method] Changes of tubulin and actin expression and types of total sperm protein were analyzed by SDS-PAGE and Western bolt after capacitation and low tempera- ture treatments were conducted in this experiment. [Result] Types of total sperm proteins changed signifi- cantly after treatment of low temperature, while capacitation did not change the proteins. The expression level of cytoskeletal tubulin and actin decreased after treatment of low temperature. Expression of tubulin after being frozen in --196 ℃ was higher than being frozen in --80 ℃ ,while expression of tubulin was al- most same at these two temperatures. [Conclusion] Low temperature treatments affect significantly the types of to- tal sperm proteins and expression of cytoskeletal tubulin and actin,but effects of capacitation are neglectable.

关 键 词：猪精子 获能 低温 SDS—PAGE Western BLOT 

分 类 号：S828.34[农业科学—畜牧学]