复方861合剂对肝星状细胞HSC—Tc—fos和c—jun基因表达的影响  

Inhibitory effects of Herbal compound 861 on expression of c-fos and c-jun genes in hepatic stellatecells

作　　者：王爱民[1] 阴赪宏[2] 朱志坚[1] 马红[2] 付晓霞[1] 王宝恩[2] 

机构地区：[1]解放军252医院消化科,河北保定071000 [2]北京友谊医院肝病研究中心

出　　处：《中华实验外科杂志》2013年第2期280-281,共2页Chinese Journal of Experimental Surgery

基　　金：军队“十五”杰出人才基金课题（01J002）;军队“十一五”面上课题（06MA073）

摘　　要：目的观察中药复方861合剂对肝星状细胞c．fos和c—jun基因表达的影响。方法在培养的肝星状细胞株中分别加入不同浓度的复方861合剂（终质量浓度1．00，0．50，0．25g／L）；于8、24、48、72h4个时间点分别收集细胞，提取肝星状细胞总RNA；用逆转录．聚合酶链反应（RT—PCR）方法测定c—fos和c-jun的基因表达水平。结果复方861合剂3组HSC—T细胞c—fos基因表达水平在8h（0．13±0．04、0．20±0．05、0．36-t-O．09）、24h（0．10±0．03、0．16-t-O．04、0．30±0．05）、48h（0．08±0．02、0．12±0．03、0．26±0．04）、72h（0．06±0．01、0．09±0．02、0．21±0．03）4个时间点均明显低于对照组（0．63±0．13、0．50±0．12、0．43±0．06、0．32±0．04）；c-jun基因表达水平在8h（0．20±0．04、0．36±0．06、0．53±0．10）、24h（0．17-4-0．04、0．32±0．07、0．47±0．10）、48h（0．13-t-O．03、0．30±0．06、0．40±0．08）、72h（0．10-t-O．02、0．23±0．03、0．32±0．04）4个时l司点也均明显低于对照组（0．93±0．13、0．80±0．12、0．63±0．10、0．32±0．04）。随剂量加大，HSC-T细胞c—fos、c—jun基因表达水平逐渐降低；3组间差异有统计学意义（P〈0．01）。结论复方861合剂对肝星状细胞c—fos和c—jun基因表达有明显的下调作用。Objective To investigate the effects of herbal compound 861 on the expression of c-fos and c-jun genes in hepatic stellate cells. Methods Hepatic stellate cells （HSCs） were cultured in medium containing different concentrations of compound 861 （ 1.00, 0. 50, 0. 25 g/L respectively） and harvested at different incubation periods （8, 24, 48, 72 h）. Total RNA of HSCs was isolated and the expression lev- els of c-fos and c-jun genes were detected by using reverse transcription polymerase chain reaction. Results C-fos gene expression levels of HSCs in high （1.00 g/L）, middle （0. 50 g/L） and low （0. 25 g/L） con- centrations of compound 861-treated groups were significantly lower than those in control group （0. 63 ＋ 0. 13, 0.50±0. 12, 0.43 ＋0.06, 0.32 ±0.04） after exposure to compound 861 at 8 h （0. 13 ：t：0.04, 0.20±0.05, 0.36 ±0.09）, 24 h （0. 10 ±0.03, 0. 16 ±0.04, 0.30 ±0.05）, 48 h （0.08 ±0.02, 0. 12 ±0. 03, 0. 26 ±0. 04） and 72 h （0. 06 ±0.01, 0. 09 ±0. 02, 0. 21 ±0. 03）. C-jun gene expression levels of HSCs in high, middle and low concentrations of compound 861-treated groups were also signifi- cantly lower than those in control group （0. 93 ±0. 13,0. 80 ±0. 12, 0. 63 ±0. 10, 0. 32 ：tO. 04） after ex- posure to compound 861 at 8 h （0. 20 ±0. 04, 0. 36 ±0. 06, 0. 53 ±0. 10）, 24 h （0. 17 ±0.04, 0. 32 ± 0. 07, 0. 47 ＋0. 10）, 48 h （0. 13 ±0. 03, 0. 30 ±0. 06, 0.40 ±0.08） and 72 h （0. 10 ±0. 02, 0. 23 ± 0. 03, 0. 32 ±0. 04）. The expression levels of c-fos and c-jun genes in HSCs were decreased gradually as the dosage of compound 861 increased, and there were significant differences in the expression levels of c- fos and c-jun genes among three compound 861-treated groups. Conclusion Compound 861 may strongly down-regulate the exlaression of c-los and c-iun ~enes in HSCs.

关 键 词：复方861合剂 肝星状细胞 C-FOS C-JUN 基因表达 

分 类 号：R285.5[医药卫生—中药学]