不同浓度血小板衍生因子BB和碱性成纤维细胞生长因子对人肌腱细胞增殖和胶原形成的影响  

Effects of platelet derived growth factorB~ and basic fibroblast growth factor on proliferation andcollagen synthesis of tenocytes

作　　者：邱轶伟[1] 姜月梅[2] 张鑫[3] 张鹏[3] 朱理玮[1] 

机构地区：[1]天津医科大学总医院普通外科,300052 [2]天津医科大学总医院心血管外科,300052 [3]天津医科大学总医院分泌实验室,300052

出　　处：《中华实验外科杂志》2013年第2期348-350,共3页Chinese Journal of Experimental Surgery

基　　金：英国牛津大学Nuffield骨科中心骨科、风湿科以及肌肉关节科学系NIHR.BRU基金资助项目;天津市卫生局科研基金资助项目（2011KZll8）;天津市教委科研基金资助项目（20110121）

摘　　要：目的观察低浓度血清条件下不同浓度的血小板衍生因子BB（PDGBB）和碱性成纤维细胞生长因子（bFGF）在人肌腱细胞体外培养中对人肌腱细胞的增殖和胶原形成的影响。方法在含0％和1％胎牛血清（FBS）的a—MEM培养基中，加入PDGFBB（5、10、50ug/L），bFGF（5、10、50ug/L）进行人肌腱细胞培养，10％FBS的培养组作为对照组。使用拉马拉蓝测定细胞增殖速度及天狼猩红染色定量肌腱细胞胶原产生。结果培养基内加入50ug/LPDGFBB＋50ug／LbFGF可以把FBS的使用量降至1％，并达到10％FBS的增殖速率（约4倍增加），差异无统计学意义（P〉0．05）；50ug／LPDGFBB＋50UG/LbFGF明显抑制肌腱细胞胶原形成（0．211±0．002）ug，与对照组比较[（0．485±0．068）ng]，差异有统计学意义（P〈0．05）。结论使用50ug/LPDGFBB＋50ug／LbFGF添加于d—MEM培养基，可以把FBS使用量降至1％，不但可以达到10％FBS的增殖速率，还能够抑制肌腱细胞的胶原产生。Objective To evaluate the effect of platelet derived growth factor （PDGF）BB and basic fibroblast growth factor （bFGF） on proliferation and collagen synthesis of tenocytes in in vitro culture. Methods Human tenocytes were cultured in （x-MEM medium supplemented with fatal bovine serun （FBS） at various concentrations and both PDGFBB and bFGF. AlamarBlue was used to examine proleration of tenocytes, and Sirius red staining was employed to evaluate the collagen synthesis of the ceils studied. Results The tenocytes cultured for 14 days with 1% FBS ＋ 50 ug/L PDGFBB ＋50 uxg/L bFGF showed similar proliferation in comparison with those cultured in 10% FBS （approximately 400% increase）. Teno- cytes cultured in 50 ug/L PDGFBs ＋ 50 ug/L bFGF showed significantly reduced collagen synthesis [ （0. 211 -＋0. 002） ngl in comparison with those cnhured in 10% FBS [ （0. 485 ＋0. 068） ng]. Conclu- sion These findings have demonstrated that in the presence of 50 ug/L PDGFBB ＋ 50 ug/L bFGF in the culture medium, FBS usage can be reduced to 1%, which can not only obtain the same prolifertion rate as the 10% FBS, but also inhibit collagen synthesis.

关 键 词：肌腱细胞 血小板衍生因子 碱性成纤维细胞生长因子 增殖 胶原et derived^rnwth fhPtnr一一and ha^ie fihrnhla^t prnwth fgetnr nn nraliferafinn an— 

分 类 号：R6[医药卫生—外科学]