ApoE-／-小鼠颈总动脉粥样硬化动物模型的建立  

Establishment of an experimental model for carotid atherosclerosis in ApoE -/- mice

作　　者：余飞[1] 黄东雅[1] 堵翠[1] 刘榕[1] 

机构地区：[1]上海同济大学附属东方医院神经内科,200120

出　　处：《中华实验外科杂志》2013年第2期401-402,共2页Chinese Journal of Experimental Surgery

基　　金：同济大学人才建设项目（1500144001）

摘　　要：目的探讨建立载脂蛋白E（ApoE-／-）小鼠颈动脉粥样硬化动物模型的有效方法。方法C57BL／6小鼠3只，ApoE-／-小鼠6只，共分为3组。A组：C57BL／6小鼠（n=3）给予普通饲料喂养；B组：ApoE。小鼠（n：3）给予高脂饲料喂养；C组：ApoE-／-小鼠（n=3）给予高脂饲料结合颈动脉部分结扎术。1周后采用超声检测c组ApoE-／-小鼠左、右颈总动脉中点的收缩期峰值流速（PSV）和血流方向；5周后检测血脂，油红O染色观察各组小鼠左颈总动脉粥样斑块形成。结果C组ApoEI／一小鼠术后1周左颈总动脉PSV为（9．4±0．8）cm／s，较右颈总动脉PSV（102．0±7．8）cm／s显著减少（P〈0．01），同时舒张期出现反向血流；ApoE-／-小鼠在高脂喂养5周后出现明显高脂血症；油红O染色可见C组ApoE-／-小鼠形成进展型纤维粥样斑块。结论颈总动脉部分结扎术加高脂饮食法建立ApoE-／-小鼠颈总动脉粥样硬化模型可有效缩短造模时间。Objective To levelop a model of-atherosclerosis in apolipoprotein E （ApoE-/- ） mice. Methods Totally 3 male C57BL/6 mice and 6 ApoE-/- mice were divided into 3 groups： C57BL/6 mice （n =3） in group A were fed on a chow diet for5 weeks; ApoE-/ mice （n =3） in group B were fed on a high fat diet for 5 weeks; ApoE -/- mice （n = 3 ） in group C were subjected to partial ligation of carotid artery followed by feeding of a high-fat diet for 5 weeks. The flow velocity at the midpoint of the common carotid arteries in group C was measured by animal ultrasonogram at 1 st week after operation. The venerous blood was taken for measuring the level of serum lipid before the mice were sacrificed after 5 weeks. The left carotid artery in all mice was quickly removed for histological examination. Results Peak systolic ve- locity （PSV） in LCA [ （9. 4 ＋0. 8） cm/s] was significantly slower than in RCA [ （102.0 _＋7. 8） cm/s] during systole in group C （P 〈0. 01 ） at first week after partial ligation, and during diastole reversed flow in LCA was observed. Hyperlipidemia was observed in ApoE -/- mice fed on high fat diet for 5 weeks. Oil O red staining showed that partial ligation and high fat diet induced advanced fibrous atheroma plaque in LCA of apoE）/- mice. Conclusion The novel combined methods of high diet with partial ligation of carotid artery can be used to establish a successful atherosclerotic animal model.

关 键 词：载脂蛋白E 颈总动脉部分结扎术 动脉粥样硬化 

分 类 号：R54[医药卫生—心血管疾病] R-332[医药卫生—内科学]