急性梗阻性化脓性胆管炎患者胆汁及血清中肿瘤坏死因子α、白细胞介素-6水平分析  

Relation of TNF-alpha and IL-6 in serum and bile of patients with acute obstructive suppurative cholangitis

作　　者：魏国强[1] 付汉中 王仲玉[1] 李仲启[1] 李彩霞[1] 马正尧 满兴山[2] 

机构地区：[1]嘉峪关市酒钢医院消化内科,甘肃嘉峪关735100 [2]嘉峪关市酒钢医院检验科,甘肃嘉峪关735100

出　　处：《临床肝胆病杂志》2013年第1期75-76,81,共3页Journal of Clinical Hepatology

摘　　要：目的通过研究急性梗阻性化脓性胆管炎(AOSC)患者胆汁及血清中肿瘤坏死因子(TNF)α和白细胞介素(IL)-6的含量,探讨二者在此病中的表达水平及临床意义。方法经内镜逆行胰胆管造影(ERCP)检查确诊AOSC 30例,采用双抗体夹心酶联免疫吸附测定(ELISA)试剂盒检测胆汁和血清中TNFα、IL-6的含量,同时与40例非AOSC病例对照。并于术后第4 d经鼻胆管引流测定30例AOSC患者胆汁中TNFα、IL-6的含量。结果 AOSC组术中胆汁中TNFα、IL-6含量分别为(179.25±26.50)、(207.00±42.05)pg/ml,血清中TNFα、IL-6含量为(189.14±28.89)、(420.18±89.04)pg/ml。对照组胆汁中TNFα、IL-6含量为(4.02±2.17)、(7.48±3.89)pg/ml,血清中TNFα、IL-6含量为(8.16±2.29)、(14.92±4.73)pg/ml。AOSC组胆汁和血清TNFα、IL-6含量高于对照组(P<0.01),AOSC组患者术后第4 d与术中胆汁TNFα、IL-6比较明显下降(P<0.01)。结论 AOSC组患者胆汁和血清中TNFα、IL-6含量高于对照组,TNFα、IL-6可能参与了AOSC的病理生理过程且可作为诊断AOSC的参考指标。Objective To investigate the dynamic changes in tumor necrosis factor-alpha （TNF α） and interleukin -6 （IL -6） levels in bile and serum of patients with acute obstructive suppurative cholangitis （ AOSC ） and evaluate the clinical significance of these factors. Methods Thirty patients diagnosed with AOSC by endoscopic retrograde cholangiopancreatogram （ERCP） and 40 patients classified as non - AOSC by ERCP （controls） were enrolled in the study. The double - antibody sandwich enzyme - linked immunosorbent assay was used to measure TNF α and IL - 6 content in bile and serum samples taken at study enrollment （ baseline, for AOSC and non - AOSC groups） and four days after surgery （ for AOSC group）. Results The AOSC group showed significantly higher baseline content of TNF α （ bile ： 179. 25± 26.50 pg/ml, serum ： 189.14 ± 28.89 pg/ml） and IL - 6 （ bile ： 207 ± 42.05 pg/ml, serum ： 420.18 ± 89.04 pg/ml） than the control group （TNF α bile： 4.02 ±2.17 pg/ml, serum： 8.16 ±2.29 pg/ml; IL-6 bile： 7.48 ±3.89 pg/ml, serum： 14.92 ±4. 73 pg/ml） （all P 〈 0.01 ）. In addition, the AOSC group showed significantly reduced levels of TNF α and IL -6 in bile on the fourth day after surgery （vs. baseline, P 〈 0.01 ）. Conclusion AOSC patients have enhanced levels of TNF α and IL - 6 in both serum and bile, suggesting that these two inflammation - related cytokine factors may be involved in the disease pathophysiology. Future studies should investigate whether TNF α and IL -6 can be used as diagnostic markers of AOSC.

关 键 词：急性梗阻性化脓性胆管炎 肿瘤坏死因α 白细胞介素6 

分 类 号：R657.4[医药卫生—外科学]