手性HPLC测定Naftopdil代谢产物的对映体  

Determination of Naftopidil metabolites enantiomers by Chiral HPLC

作　　者：白浩[1] 刘夏雯[1] 袁牧[1] 

机构地区：[1]广州医学院药物研究中心,广东广州510182

出　　处：《华西药学杂志》2013年第1期89-91,共3页West China Journal of Pharmaceutical Sciences

基　　金：2010年广州市科技专项(项目编号:2010U1-E00531-2)

摘　　要：目的分离和测定Naftopidil手性代谢产物:去甲基代谢产物(DMN)、苯羟化代谢产物(PHN)和萘羟化代谢产物(NHN)的对映体。方法采用HPLC法,流动相为异丙醇-正己烷(15∶85),流速为1.0 mL.min-1,柱温为25℃,检测波长为283 nm。结果 3对对映体能同时得到良好地分离。经方法学验证,R-DMN(4.99～159.68μg.mL-1)、R-PHN(5.30～168.00μg.mL-1)、R-NHN(5.00～160.16μg.mL-1)与峰面积线性良好;RSD<1.03%。结论所用方法简单、快速、准确,可同时测定合成的R-DMN、R-PHN和R-NHN的含量,为Naftopidil手性药物代谢学研究提供了方法。OBJECTIVE To separate and determine enantiomers of Naftopidil metabolites desmethyl naftopidil（DMN）,phenylhydroxy naftopidil（PHN）and naphthylhydroxy naftopidil（NHN） by chiral HPLC.METHODS The enantiomers of DMN,PHN and NHN were separated and monitored at the wavelength of 283 nm.The mobile phase consisting of isopropanol-hexane（15：85） was pumped at a flow rate of 1.0 mL·min-1and the column temperature was set at 25 ℃.RESULTS Under such conditions,the enantiomers were completely separated in a simultaneous injection.Validated method had excellent linearity in the range of R-DMN（4.99-159.68 μg·mL-1）and R-PHN（5.30-168.00 μg·mL-1）and R-NHN（5.00-160.16 μg·mL-1）,RSD1.03%.CONCLUSION The validated method was simple,rapid and reproducible for stereoselective separations and qualifications of naftopidil metabolites enantiomers.

关 键 词：NAFTOPIDIL 手性代谢产物 手性高效液相色谱法 对映异构体 含量测定 

分 类 号：R94[医药卫生—药剂学]