成纤维细胞激活基因的调控及改善皮肤老化的研究  

Regulation of activated genes of fibroblasts and their improvement of skin aging

作　　者：亓玉青[1] 李天航[1] 范丽云[1] 刘全忠[1] 

机构地区：[1]天津医科大学总医院皮肤科,300052

出　　处：《中华医学美学美容杂志》2013年第1期43-46,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的通过研究雌二醇、维生素E、维生素C对体外培养的人皮肤成纤维细胞丛质金桶蛋白酶1（MMP1）mRNA、透明质酸合成酶-2（HAS-2）mRNA转录水平的影响，探训小Ⅲ药物改善皮肤老化的叮能机制。方法将不同浓度的雌二醇、维生素E、维生素C分别加入体外培养的人皮肤成纤维细胞，逆转录-聚合酶链反应（RT—PCR）榆测不同物质对成纤维细胞中HAS-2mRNA、MMt）。1mRNA表达水平的影响。结果雌二醇、维生素C对MMP-1mRNA表达的影响：高剂量组、低剂量组与对照组的差异有统计学意义（P〈0．05）。低剂量组与高剂量组差异无统计学意义（P〉0．05）。维生素E、维生素C对HAS-2mRNA表达的影响：高剂量组、低剂量组与对照组的差异有统计学意义（P〈0．05），低剂量组与高剂带组差异无统计学意义（P〉0．05）。结论雌二醇、维生素C可以下调MMP-1基因的转录水平，减少后者对皮肤胶原的降解；维生素E、维生素C可以上调HAS-2基因的转录水平，可能增加透明质酸的合成，从而改善皮肤老化。Objective To investigate the possible mechanisms using different chemicals to im- prove the skin aging by the effect of estrogen, vitamin E, vitamin C on the transcriptional level of MMP-1 and HAS-2 mRNAs in cultured human skin fibroblast in vitro. Methods The different con-centrations of estrogen, vitamin E, vitamin C were put into cultured human skin fibroblasts. After 24 hours, RT-PCR was used to detect the effects of the different chemicals on the transcriptional levels of the HAS-2 and MMP-1 mRNAs in fibroblasts. Results The effects of estrogen and vitamin C on the MMP-1 mRNA had significant difference between the high dose group and blank control group, and between the low dose group and blank control group （P〈0.05）; the difference between the low dose group and high dose group was not statistically significant （P〉0.05）. The effects of vitamin E and vi-tamin C on Has 2 mRNA also had significant difference between the high dose group and the blank control group, and between the low dose group and blank control group （P〈0.05）, but the difference between the low dose group and high dose group was not statistically significant （P〉0.05）. Conclu-sions Estrogen and vitamin C can reduce the transcription of MMP-1 gene, which decreases the deg-radation of collagen in the skin; vitamin E and vitamin C can increase the transcription of HAS-2 gene, which may possibly increase the synthesis of hyaluronic acid to alleviate the skin aging.

关 键 词：基质金属蛋白酶1 透明质酸合成酶-2 成纤维细胞 皮肤老化 

分 类 号：R622[医药卫生—整形外科]