前列腺素El影响扩张皮瓣存活的干预机制  

Interference mechanism of liposome PGEl on survival of expanded flap

作　　者：李薇薇[1] 刘志飞[2] 乔群[2] 王晓军[2] 赵茹[2] 朱琳[2] 

机构地区：[1]清华大学附属北京清华医院整形外科,100218 [2]北京协和医院整形外科

出　　处：《中华医学美学美容杂志》2013年第1期47-50,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的观察脂质体携载前列腺素El（PC-El）埘扩张皮瓣存活的干预机制。方法采用中国小型猪为实验动物，实验分2组：脂质体携载PGEl组（实验组）及未用药对照组。在其背部形成8cm×2cm扩张皮瓣，通过图像分析技术及Real—time PCR技术，观察脂质体携载PGEl肿皮瓣存活的影响及作用机制。结果实验组与对照组相比，皮瓣存活率增高（P〈0．01），血管内皮细胞牛长因子（VEGF）、iNOS、Ang-1基冈表达均降低，β1转化生长因子（TGF-β1）基冈表达增高，但组问差异无统计学意义（P〉0．05）。结论应用脂质体携载PGEl可以提高扩张皮瓣存活率．但脂质体携载PGEl减轻扩张皮瓣缺血一再灌注损伤的机制不是通过调声VEGF、iNOS、Ang-1及TGF-β1基因的表达实现的。Objective To investigate the mechanism of liposome PGEl on the survival of the ex panded flap. Methods The minipigs were set up as an animal model. They were divided into 2 groups. The expanded flaps （8 cm ×2 cm） were designed and elevated on the back of an minipig. An image analysis technology was used to evaluate the survival rate of the expanded flap, while reahime-PCR technique was used to investigate the molecular mechanism. Results The survival area of the ex-panded flap in the l.ipo PGEl group was significantly enlarged （P〈0.01） compared with the control group. The VEGF, iNOS and Ang-1 gene expression decreased, but TGF-β1 gene expression in-creased. But there was no significant difference between two groups （P〉0.05）. Conclusions The Lipo PGEl could promote survival rate of the expanded flap, but the mechanism does not involve the regulation of VEGF, iNOS, Ang-1 and TGF-β1 gene expression.

关 键 词：前列腺素E1 扩张皮瓣 干预机制 

分 类 号：R622[医药卫生—整形外科]