胱天蛋白酶-8在UVB诱导人皮肤成纤维细胞凋亡中的作用  

The role of caspase-8 in the apoptosis of dermal fibroblasts induced by ultraviolet B irradiation

作　　者：徐浩翔[1] 李海玲[2] 闫言[3] 李莉[4] 王宝玺[1] 

机构地区：[1]中国医学科学院皮肤病研究所,南京210042 [2]河南省新乡市获嘉县人民医院皮肤科,453800 [3]中国医学科学院北京协和医学院皮肤科,北京100730 [4]首都医科大学附属北京朝阳医院皮肤科,北京100043

出　　处：《中国麻风皮肤病杂志》2013年第1期8-10,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金(编号:81101207);教育部高校博士点基金(编号:20111106120052)

摘　　要：目的:确定胱天蛋白酶-8(caspase-8)在UVB诱导皮肤成纤维细胞凋亡中的作用。方法:人皮肤成纤维细胞经100、200、300和400 mJ/cm^2 UVB照射后48 h,用MTT法检测细胞活性变化,用Hoechst33258染色法检测细胞凋亡,并用抑制剂Z-IETD-FMK抑制caspase-8活性后,检测凋亡细胞数量。结果:UVB照射明显抑制成纤维细胞活性,引起细胞凋亡,随着照射UVB剂量的增加,凋亡细胞逐渐增多。Caspase-8抑制剂Z-IETD-FMIK抑制UVB照射引起的细胞凋亡。结论:caspase-8在UVB照射导致的UVB急性光损伤中发挥重要作用。Objective： To determine the role of caspase- 8 in the apoptosis of human skin fibroblasts induced by UVB irradiation. Methods： Primary human skin fibroblasts were cultured and were irradiated with 100, 200, 300 and 400 mJ/cm^2 UVB. The viability of the cells was measured by MTT assay at 48 hours after irradiation. The apoptosis of the fihrohlasts was measured with Hoeehst 33258 staining, and was re - measured after the viability of caspase - 8 was inhibited by adding of Z - IEFD - FMK inhibitor. Results： The UVB irradiation markedly inhibited the viability of human skin fibroblasts and induced apoptosis in a dose - dependent manner. After adding of Z - IETD - FMK （the caspase - 8 inhibitor）, the apoptosis induced by UVB irradiation was inhibited. Conclusion： Caspase - 8 may play an important role in the process of acute photodamage.

关 键 词：紫外线B 人皮肤成纤维细胞 凋亡 胱天蛋白酶-8 

分 类 号：R751[医药卫生—皮肤病学与性病学]