野生稻渗入系颖壳颜色的简易测定及QTL定位分析  

DETECTION OF GLUME COLOR OF WILD INTROGRESSION LINES AND QTL ANALYSIS

作　　者：万国峰[1] 吕志伟[1] 王春明[1] 侯雷[1] 李楠[1] 赵志超[2] 张文会[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《核农学报》2013年第1期15-20,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金资助项目(30771156);山东省自然科学基金资助项目(2005ZX10;2009ZRB01762);教育部留学回国科研启动资助项目(2005)

摘　　要：用Unispec光谱仪测定水稻颖壳反射光谱,筛选对水稻颖壳色素敏感的色素指数,用筛选出的最佳植被指数NDVI作为检测颖壳颜色的指标,测定106个家系的颖壳颜色用于QTL定位分析。共检测到12个与颖壳颜色相关的QTL,其中有4个来源于栽培稻特青,分别位于第1染色体RM243附近,贡献率为5%;第7染色体RM295、RM481和RM82附近,贡献率分别为4%、7%和4%。另外8个QTL位点来源于野生稻,分别位于第1染色体RM5和RM212附近,贡献率分别为5%和6%,第2染色体RM233A附近,贡献率为6%;第4染色体RM273附近,贡献率为38%;第6染色体RM204和RM3附近,贡献率分别为17%和5%;第8染色体RM38附近,贡献率为6%,以及位于第12染色体RM235附近,贡献率为5%。在检测到的12个QTL中,来源于野生稻的位于第4染色体RM273附近,以及位于第6染色体RM204附近的QTL的加性效应及贡献率较大,分析是主效QTL。In order to screen the sensitive wavelength which related to rice hull color index,we used Unispec spectrometer to survey reflectance spectra of rice glume,then chose the optimal NDVI to predict rice hull color expressed as hull color index.Rice hull color index from 106 rice families were determined for QTL analysis.We detected twelve QTLs for rice glume color.Among four QTLs with positive allele came from cultivated rice Teqing,one was located nearby the markerRM243 on chromosome 1,explained 5% of phenotypic variation;Others were located nearby RM295,RM481 and RM82 on chromosome 7,explaining 4%,7% and 4% of variation,respectively.The other eight QTL,with positive allele came from wild rice parents,were located nearby RM5 and RM212 on chromosome 1,RM233A on chromosome 2,RM273 on chromosome 4,RM204 and RM3 on chromosome 6,RM38 chromosome 8,and RM235 on chromosome 12,respectively.Two of them,RM273 and RM204,displayed large,additive effect and explained large phenotypic variation,they might be major QTL.

关 键 词：水稻 颖壳颜色 NDVI QTL 

分 类 号：S511.9[农业科学—作物学]