虫草多糖对二甲基亚硝胺诱导的肝纤维化大鼠脂质过氧化及肝细胞再生的影响  

Effect of Cordyceps polysaccharide on lipid peroxidation of rats with dimethylnitrosamine-induced liver fibrosis

作　　者：彭景华[1] 李雪梅[1,2] 胡义扬[1] 冯琴[1] 

机构地区：[1]上海中医药大学附属曙光医院上海中医药大学肝病研究所肝肾疾病病证教育部重点研究室上海市高校中医内科学E-研究院,上海201203 [2]天津中医药大学第一附属医院,天津300192

出　　处：《中国中药杂志》2013年第3期391-396,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(30973924);国家"重大新药创制"科技重大专项子课题(2012ZX09103201-048);教育部博士点基金(2007268002);上海市教委科研创新项目(08ZZ64);上海市教委创新团队(第1期);国家中医药管理局中医肝胆病重点学科项目

摘　　要：目的:观察虫草多糖对二甲基亚硝胺(DMN)诱导的大鼠肝纤维化的药理作用。方法:采用DMN大鼠肝纤维化模型,分为正常组(N,n=6)、模型组(M,n=11)、虫草多糖组(C,n=8)、秋水仙碱组(Q,n=9)。造模4周,虫草多糖(60 mg.kg-1)和秋水仙碱(0.1 mg.kg-1)灌胃给药3周,模型与正常组同时给予等量灭菌水。观察血清ALT,AST,GGT活性及Alb,TBil含量;肝组织羟脯氨酸(Hyp)含量;肝脏病理及胶原染色;肝组织SOD活性及MDA,GSH,GSH-Px含量;肝脏组织增殖细胞核抗原(PCNA)蛋白表达。结果:模型组血清ALT,AST,GGT,TBil显著升高,Alb显著降低,虫草多糖组血清AST,TBil显著降低,Alb显著升高;模型组肝组织Hyp显著升高,虫草多糖、秋水仙碱组则显著降低;HE染色:模型组正常肝小叶结构破坏,肝细胞水肿,汇管区结缔组织增生,虫草多糖及秋水仙碱组上述病理变化显著减轻。胶原染色:模型组肝小叶纤维间隔形成,见较多完整假小叶;虫草多糖、秋水仙碱组胶原沉积减少。模型组肝组织SOD,GSH-Px,GSH显著降低,MDA显著增高,虫草多糖及秋水仙碱组GSH,GSH-Px活性显著增高。模型组肝组织PCNA蛋白表达显著升高,虫草多糖及秋水仙碱组则显著降低。结论:虫草多糖可显著抑制DMN诱导的大鼠肝纤维化及脂质过氧化损伤。Objective： To observe the pharmacological effect of Cordyceps polysaccharide on dimethylnitrosamine （DMN）-in- duced liver fibrosis in rats. Method： DMN rat liver fibrosis model was established and divided into the normal group （N, n = 6） , the model group （ M, n = 11 ）, the Cordyceps polysaccharide group （ C, n = 8 ） and the colchicine group （ Q, n = 9 ）. During the modeling for four weeks, Cordyceps polysaccharide （60 mg kg- i ） and colchicine （0. 1 mg kg 1 ） were orally administered for three weeks, while the model and normal groups were given disinfected water of the same amount. Observation： serum ALT, AST, GGT and Alb, TBil content; content of hydroxyproline （Hyp） in liver tissues; liver pathology and collagen staining; SOD activity and MDA, GSH, GSH-Px in liver tissues; protein expression of proliferating cell nuclear antigen （PCNA） in liver tissues. Result： Serum ALT, AST, GGT, TBil significantly increased, and Alb decreased significantly in the model group. Hepatic Hyp significantly increased in the mod- el group, whereas the index remarkably decreased in the Cordyceps polysaeeharide group and the colchicine group. HE staining： the structure of normal hepatic lobules was damaged, with hepatocytes tumefaction and proliferation of connective tissues in portal tracts in the model group, while the Cordyceps polysaccharide group and the colchicine group recorded notable reduction in above pathological changes. Collagen staining： the model group showed hepatic lobule fibrous septum and many intact pseudolobules; while the Cordyceps polysaccharide group and the colchicine group witnessed decrease in collagen deposition. The model group showed significant decrease in SOD, GSH-Px and GSH and increase in MDA, whereas the Cordyceps polysaccharide group and the colchicine group recorded nota- ble growth in GSH and GSH-Px. The model group showed significant decrease in protein expression of PCNA in liver tissues, while the Cordyceps polysaccharide group and the colchicin

关 键 词：虫草多糖 肝纤维化 脂质过氧化 肝细胞再生 

分 类 号：R285.5[医药卫生—中药学]