不同配伍戊己丸的黄连生物碱在人源肠道菌群中的代谢研究  

Study on metabolism of Coptis chinensis alkaloids from different compatibility of Wuji Wan in human intestinal flora

作　　者：门薇[1] 陈颖[2] 杨庆[2] 李玉洁[2] 巩仔鹏[2] 翁小刚[2] 王娅杰[2] 张瑞杰[1] 朱晓新[1,2] 

机构地区：[1]首都医科大学,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2013年第3期417-421,共5页China Journal of Chinese Materia Medica

基　　金：国家科学自然基金重点项目(30930114);国家"重大新药创制"科技重大专项(2009ZX09301-005-09);中国中医科学院基本科研业务费自主选题项目(ZZ20090101;ZZ20070406)

摘　　要：目的:研究戊己丸配伍方中的小檗碱、巴马汀在人源肠道菌群中的代谢。方法:采用L9(34)正交设计法将黄连、吴茱萸、土炒白芍组成9组戊己丸配伍方和相应剂量的黄连单味药,分别与人的新鲜粪便进行厌氧培养,在培养24 h后采用HPLC-UV法同时测定菌液中小檗碱和巴马汀的含量,比较在不同配伍比例下2种成分的代谢。结果:在黄连提取物中,小檗碱的代谢速度与剂量呈正相关,巴马汀的代谢速度与剂量呈负相关。复方配伍加速了低剂量小檗碱的代谢,与吴茱萸和土炒白芍呈正相关;而减慢了高剂量小檗碱的代谢,代谢速度与吴茱萸呈负相关。复方配伍加速了高剂量巴马汀的代谢,减慢了低剂量巴马汀的代谢,并且代谢速度与吴茱萸和土炒白芍的剂量呈负相关。结论:当黄连代表成分在肠道菌群中代谢较快时,戊己丸复方配伍减慢其代谢速度,而当黄连代表成分在肠道菌中代谢缓慢时,戊己丸复方配伍则加快其代谢,从而起到平衡的作用。而且黄连、吴茱萸、土炒白芍比例不同,小檗碱和巴马汀的代谢速度也有一定的差异。Objective ： To study the metabolism of berberine and palmatine in prescription compatibility of Wuji Wan in human intestinal flora. Method ： The L9 （ 34 ） orthogonal design was adopted to compare prescription compatibility of nine groups of Wuji Wan composed of Coptis chinensis, Evodiae and fried Radix paeoniae alba into and single ingredient of C. chinensis. They were cultivated with fresh human excrements under anaerobic conditions for 24 h. A HPLC-UV method was adopted for determining berberine and pal- marine in bacteria culture medium, in order to compare the metabolism of the two components in different prescription compatibility. Result： Metabolism of berberine was positively correlated with doses, whereas metabolism of palmatine was negatively correlated with doses in extracts from C. chinensis. Compound compatibility speeded up the metabolism of berberine in low dose, which was positively related to the doses of Evodiae and fried Paeoniae Alba Radix ; meanwhile Compound compatibility slowed down the metabolism of ber- berine in high dose, which was negatively related to the dose of Evodiae. Compound compatibility speeded up the metabolism of palma- fine in high dose, which was negatively related to the doses of Evodiae and fried Paeoniae Alba Radix. Conclusion： The metabolism of the compatibility of Wuji Wan speeds up, when Coptis chinensis components metabolite rapidly in intestinal flora ; while the metabolism of the compatibility of Wuji Wan slows down, when C. chinensis components metabolite slowly in intestinal flora. Therefore, they show a balanced effect. Additionally, different proportion of C. chinensis, Evodiae and fried Paeoniae Alba Radix cause difference in metab- olism speed of berberine and palmatine to some extent.

关 键 词：肠道菌群 戊己丸 小檗碱 巴马汀 

分 类 号：R285[医药卫生—中药学]