超滤法测定牛蒡苷及牛蒡苷元的血浆蛋白结合率  

Determination of plasma protein binding rate of arctiin and arctigenin with ultrafiltration

作　　者：韩雪莹[1] 王巍[1] 谭日秋[1] 窦德强[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国中药杂志》2013年第3期432-434,共3页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-423);沈阳市科技局科学计划项目(F11-264-1-20)

摘　　要：目的:测定牛蒡苷及牛蒡苷元的血浆蛋白结合率。方法:采用超滤法结合高效液相色谱法对牛蒡苷及牛蒡苷元与大鼠血浆以及健康人血浆蛋白结合率进行测定。结果:牛蒡苷与大鼠血浆在64.29,32.14,16.07 mg.L-1下的血浆蛋白结合率分别为(71.2±2.0)%,(73.4±0.61)%,(78.2±1.9)%,与健康人血浆的血浆蛋白结合率分别为(64.8±3.1)%,(64.5±2.5)%,(77.5±1.7)%;牛蒡苷元与大鼠血浆在77.42,38.71,19.36 mg.L-1下的血浆蛋白结合率分别为(96.7±0.41)%,(96.8±1.6)%,(97.3±0.46)%,与健康人血浆的血浆蛋白结合率分别为(94.7±3.1)%,(96.1±0.3)%,(97.9±1.3)%。结论:牛蒡苷与大鼠血浆蛋白有中等强度的结合,略高于与人血浆蛋白结合率;牛蒡苷元与大鼠血浆和健康人血浆均有很强的结合。Objective： To determine the plasma protein binding rate of arctiin and arctigenin. Method： The uhrafihration combined with HPLC was employed to determine the plasma protein binding rate of arctiin and arctigenin as well as rat plasma and heahhy human plasma proteins. Result： The plasma protein binding rate of arctiin with rat plasma at the concentrations of 64. 29, 32. 14, 16. 07 mg L-1 were （71.2 ±2. 0）%, （73.4 ±0. 61 ）%, （78. 2 ± 1.9）%, respectively; while the plasma protein binding rate of arctiin with healthy human plasma at the above concentrations were （64. 8 ± 3. 1 ） %, （64. 5 ± 2. 5 ） %, （ 77. 5± 1.7 ） %, re- spectively. The plasma protein binding rate of arctigenin with rat plasma at the concentrations of 77.42, 38.71 , 19.36 mg L-1 were （96. 7 ± 0. 41 ） %, （96. 8 ±1. 6） %, （97. 3 ± 0. 46 ） %, respectively ; while the plasma protein binding rate of arctigenin with normal human plasma at the above concentrations were （94. 7±3. 1 ） %, （96. 8± 1.6） %, （97. 9 ±1. 3） % , respectively. Conclusion： The binding rate of arctiin with rat plasma protein was moderate, which is slightly higher than the binding rate of arctiin with healthy human plasma protein. The plasma protein binding rates of arctigenin with both rat plasma and healthy human plasma are very high.

关 键 词：牛蒡苷 牛蒡苷元 血浆蛋白结合率 超滤法 高效液相色谱法 

分 类 号：R285[医药卫生—中药学]