Toll样受体2(TLR2)参与猪链球菌诱导的自噬作用  

Toll-like receptor 2 is involved in autophagy induced by Streptococcus suis type 2

作　　者：李文华[1] 王莹[1] 陈伟[1] 黄清花[1] 陈福广[1] 张巧灵[1] 刘波[1] 杨振国[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2013年第2期222-226,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972170);科学前沿与交叉学科创新资助项目(200903337)

摘　　要：为证实Toll样受体2(TLR2)是否参与2型猪链球菌诱导的细胞自噬作用,通过阻断TLR2,并建立2型猪链球菌感染J774A.1细胞模型,分别应用荧光定量PCR分析不同处理组细胞TLR2和NF-κB mRNA水平、ELISA检测细胞上清中TNF-α和IL-6含量变化以及Western blot检测细胞内LC3蛋白的表达量。结果显示,TLR2和NF-κBmRNA水平在感染后3h明显高于对照组;与对照组相比,感染组TNF-α含量及LC3Ⅱ表达均明显升高(P<0.01);与感染组相比,阻断TLR2后TNF-α含量及LC3Ⅱ表达均明显降低(P<0.05)。结果表明,2型猪链球菌可激活TLR2及其信号通路,并且该通路的活性影响细胞自噬作用。In order to confirm whether TLR2 involved in autophagy induced by Streptococcus suis type 2,J774A. 1 cells pretreated with anti-TLR2 antibody were infected by Streptococcus suis type 2 in vitro. The transcriptional levels of TLR2 and NF-κB mRNA were analysised by real-time PCR,then ELISA and Western blot were used to detect the expression of TNF-Ⅱ,IL-6 and LC3. Compared with control group, the transcription of TLR2 and NF-κB was significantly increased at 3 h after infection, and the expression of TNF-α and LC3 Ⅱ was highly significant （P〈0.01） increased. However, compared with experimental groups, the expression of TNF-α and LC3 Ⅱ was significantly （P 〈 0. 05） decreased after blocking TLR2. The results indicated that Streptococcus suis type 2 could activate TLR2 and its signal pathway, which affected macrophage autophagy.

关 键 词：TOLL样受体2 2型猪链球菌 巨噬细胞 自噬 

分 类 号：S852.4[农业科学—基础兽医学] S855.9[农业科学—兽医学]