新疆石河子地区捕食线虫性真菌的分离鉴定  

Isolation and identification of Nematophagous fungi from Shihezi region in Xinjiang

作　　者：王为升[1] 孟庆玲[1] 乔军[1] 王俊伟[1] 罗建勋[2] 才学鹏[2] 赵春光[1] 张再超[1] 田振中[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《中国兽医学报》2013年第2期230-235,共6页Chinese Journal of Veterinary Science

基　　金：新疆建设兵团博士资金资助项目(2009JC09);石河子大学高层次人才专项资助项目(RCZX200801)

摘　　要：以山羊捻转血矛线虫第三期幼虫作为诱饵线虫,利用撒布分离法对新疆石河子地区土壤中的捕食线虫性真菌进行分离,并对所分离的捕食线虫性真菌进行了形态学及分子生物学鉴定。结果成功分离出了9株捕食线虫性真菌,其形态学符合少孢节丛孢菌。将分离株5.8SrDNA和ITS2基因序列与国内外已知少孢节丛孢菌菌株的对比分析,证明少孢节丛孢菌新疆分离株具有区域性特征,其捕食活性高于报道的菌株。分离出适合本地区生态环境的当地菌株对以后家畜线虫病的生物防控尤为必要。In the study, goat haemonchus contortus Ira was used as revulsive nematodes to isolate Nematophagousfungi by broadcast sowing mathod from soil samples from Shihezi region,Xin- jiang province. The morphological and molecular biologic characteristics were observed and detected after isolation. Nine strains of Arthrobotrys oligospora were successfully isolated from soil samples, and the morphological characteristics were identified and described. Compared with known Arthrobotrys oligospora strains,Arthrobotrys oligosporas from different geographical areas have regional characteristics based on 5.8S rDNA and ITS2 gene sequence,and the predation rates were higher than that of the reported Arthrobotrys oligospora strains. Therefore,it is important to isolate local strains which adapt to the local ecological environment for the prevention and control of livestock nematode in future.

关 键 词：捕食线虫性真菌 形态特征 捻转血矛线虫三期幼虫 

分 类 号：S855.4[农业科学—临床兽医学] S855.9[农业科学—兽医学]