ICR小鼠胰岛内nestin阳性细胞的分离培养及特性鉴定  

Isolation,culture and identification of nestin positive cells in ICR mouse islets

作　　者：张胜[1] 张鹏[1] 张学明[1] 冯怀亮[1,2] 李子义[1] 

机构地区：[1]吉林大学畜牧兽医学院动物胚胎工程吉林省重点实验室,吉林长春130062 [2]美国康乃尔大学妇产科系生殖中心

出　　处：《中国兽医学报》2013年第2期241-245,共5页Chinese Journal of Veterinary Science

基　　金：国家"973"计划重大科学问题导向资助项目(2011CBA01003)

摘　　要：通过向ICR小鼠胆总管注射胶原酶Ⅴ的方法体外分离胰岛,并从中分离培养出nestin+细胞,之后通过低糖、低血清诱导其分化产生类胰岛样细胞团。结果显示,小鼠胰岛内存在nestin+细胞,将其分离纯化后在体外低糖(2.5mmol/L)和低血清(2%)条件下可诱导分化为胰岛样细胞团;表明nestin+细胞具有胰腺干/祖细胞的特性。The islets were isolated from ICR mice by using the intraductal collagenase technique, then nestin＋ cells were separated and expanded. Those cells were induced into medium containing low glucose concentration（2.5 mmol/L） and low serum（2%）. The results indicated that mouse is- lets contain nestin＋ cells. After isolation,they can differentiate into islet-like structure at low glucose concentration（2.5 mmol/L） and low serum（2%） conditions in long-term culture,demonstra- ting that nestin; mouse islet cells may have the biological properties of pancreas precursor cells.

关 键 词：ICR小鼠 胰腺干 祖细胞 胰岛 nestin+细胞 胰岛样细胞团 

分 类 号：S811.4[农业科学—畜牧学]