姜黄素对大鼠非酒精性脂肪性肝炎的作用及其机制初探  

Effects of curcumin on nonalcoholic steatohepatitis in rats and its mechanisms

作　　者：李兵兵[1] 王龙[2] 陈维雄[2] 朱金水[2] 

机构地区：[1]苏州大学医学部,江苏215006 [2]上海交通大学附属第六人民医院消化内科

出　　处：《肝脏》2013年第1期18-22,共5页Chinese Hepatology

摘　　要：目的探讨姜黄素对高脂饮食诱导的大鼠非酒精性脂肪性肝炎(NASH)的防治作用及其机制。方法 SD大鼠36只随机分为正常组、模型组和治疗组(每组12只)。正常组全程饲以普通饲料,另两组均给予高脂饮食。6周末,每组各处死2只大鼠。验证非酒精性单纯性脂肪肝(NAFL)造模成功后,给予治疗组50mg·kg-1·d-1姜黄素灌胃,正常组、模型组给予相应体积的0.5%羧甲基纤维素钠(CMC)灌胃。6周治疗结束后,留取血样和肝组织,计算肝指数,进行血脂、肝功能和病理组织学检查;检测肝脏组织丙二醛(MDA)、谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)的活性;Western印迹检测肝脏转录因子NF-E2相关因子2(Nrf2)的水平。结果模型组大鼠肝组织出现NASH的病理特征。治疗组大鼠与模型组相比,血清三酰甘油、TC、ALT、AST及肝组织MDA水平均明显降低(0.36±0.17)mmol/L比(0.67±0.14)mmol/L,(1.01±0.06)mmol/L比(1.40±0.20)mmol/L,(43.67±3.22)U/L比(83.00±4.36)U/L,(82.50±7.42)U/L比(122.33±3.51)U/L,(0.54±0.95)nmol/mgprot比(1.57±0.03)nmol/mgprot;均P<0.01。肝组织脂肪变、炎症程度明显改善,GSH含量和SOD的活性升高(1.64±0.17)mg/gprot比(1.17±0.17)mg/gprot,(420.12±42.03)U/mgprot比(335.53±25.81)U/mgprot;均P<0.05。且该组肝组织内Nrf2蛋白表达较正常组、模型组显著升高(均P<0.01)。结论姜黄素可通过降脂、减弱氧应激,延缓大鼠从NAFL向NASH的进展,该作用可能与其诱导Nrf2在肝脏中的表达相关。Objective To investigate the prevention and treatment effects of eurcumin on high-fat diet induced nonalcoholic steatohepatitis in rats and to explore its mechanisms. Methods Thirty-six SD rats were randomly divided into normal group, model group and treatment group with twelve each. Rats in normal group were fed a standard diet while those in other two groups received a high-fat diet. Two rats from each group were executed at the end of the sixth week to detect the pathological changes. Then, rats in treatment group were gavaged with 50mg/kg of curcumin daily. Normal group and model group received an equal volume of 0.5% CMC as control. At the end of the twelfth week,all rats were executed. The serum biochemical parameters and liver histological examinations were observed. The contents of malondialdehyde （MDA）, glutathione （GSH） and the activity of superoxide dismutase （SOD） in liver were measured with corresponding methods. The protein expressions of liver nuclear factor-erythroid 2-related factor-2 （Nrf2） were determined by Western blot. Results Rats in model group reproduced the pathological features of NASH. Compared with this group, a remarkable reduction （P〈0. 01） were observed in serum TG, TC, ALT, AST and liver contents of MDA in treatment group [-（0. 36 ± 0.17） rnmol/L vs （0.67 ±0.14） mmol/L, （1.01 ± 0. 06） mmol/L vs （1.40±0.20） mmol/L, （43.67±3.22） U/L vs （83.00±4. 36） U/L, （82.50 ±7.42） U/L vs （122. 33±3.51） U/L, （0.54±0. 95） nmol/mgprot vs （1.57±0. 03） nmol/ mgprot, respectively] ;The degrees of hepatic steatosis and inflammation were significantly alleviated; the contents of GSH and the activity of SOD in liver [（1.64±0.17） mg/gprot vs （1.17±0.17） mg/gprot, （420. 12 ±42.03） U/mgprot vs （335.53±25.81） U/mgprot, respectively] were higher （P〈0.05）. The protein expressions of liver Nrf2 were significantly higher in treatment group than those in normal group and model group （P〈0. 01）. Conclusion Curcum

关 键 词：姜黄素 非酒精性脂肪性肝炎 大鼠 转录因子NF-E2相关因子2 

分 类 号：R285.5[医药卫生—中药学]