NUCB2/nesfatin-1在肥胖大鼠胃肠道组织中的表达  

Expression of nucleobindin-2/nesfatin-1 in gastrointestinal tissues of high-fat diet-induced obese rats

作　　者：李伟娜[1] 田字彬[1] 魏良洲[1] 孙桂荣[1] 张翠萍[1] 葛银林[2] 徐珞[2] 

机构地区：[1]青岛大学医学院附属医院消化内科,山东省青岛市266003 [2]青岛大学医学院,山东省青岛市266021

出　　处：《世界华人消化杂志》2013年第4期320-326,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81070305;30470642;山东省自然科学基金资助项目;No.zr2010hm066~~

摘　　要：目的:研究肥胖与正常大鼠胃肠组织NUCB2 mRNA及NUCB2/nesfatin-1蛋白的表达.方法:30只健康♂Wistar大鼠随机均分为高脂高营养性肥胖组和正常对照组,8wk后取其胃、十二指肠、小肠、结肠组织,分别采用实时荧光定量RT-PCR及免疫组织化学的方法检测NUCB2 mRNA及NUCB2/nesfatin-1蛋白的表达.结果:(1)肥胖组大鼠胃、十二指肠、小肠NUCB2 mRNA表达量分别是对照组的2.02、1.49、1.23倍(t=4.256,P=0.000;t=3.455,P=0.002;t=2.402,P=0.026),且NUCB2 mRNA表达量与大鼠Lee's指数均呈正相关(r=0.677,P=0.006;r=0.561,P=0.030;r=0.538,P=0.039);两组大鼠结肠组织NUCB2 mRNA表达差异无统计学意义(t=1.835,P=0.077);(2)两组大鼠胃黏膜中下2/3、十二指肠布氏腺及潘氏细胞、小肠潘氏细胞中均检测到NUCB2/nesfatin-1蛋白的表达;肥胖组大鼠胃NUCB2/nesfatin-1蛋白表达较对照组增多(Z=-2.955,P=0.003),表达量与大鼠Lee's指数呈正相关(r=0.677,P=0.008);肥胖组大鼠十二指肠及小肠潘氏细胞中NUCB2/nesfatin-1蛋白表达较对照组减少(Z=-2.026,P=0.043;Z=-2.648,P=0.008),表达量与大鼠Lee's指数均呈负相关(r=-0.557,P=0.031;r=-0.617,P=0.014).结论:NUCB2/nesfatin-1在大鼠胃肠道中分布广泛,肥胖大鼠胃组织NUCB2 mRNA及NUCB2/nesfatin-1蛋白表达上调、潘氏细胞NUCB2/nesfatin-1蛋白表达下调.AIM：To investigate the expression of the NUCB2 gene and NUCB2/nesfatin-1 protein in gastrointestinal tissues of obese and normal rats.METHODS：Thirty male Wistar rats were randomly divided into two groups to be fed a highfat diet or a normal diet.At the end of eight weeks,gastric,duodenal,small intestinal and colonic tissues were collected to detect the expression of NUCB2 mRNA and NUCB2/nesfatin-1 protein by real-time RT-PCR and immunohistochemistry,respectively.RESULTS：The expression levels of NUCB2 mRNA in gastric,duodenal,and small intestinal tissues of obese rats were 2.02,1.49 and 1.23 times higher than those in corresponding tissues of normal controls（t=4.256,3.455,2.402;P=0.000,0.002,0.026）,showing a significant positive correlation with Lee＇s index（r=0.677,0.561,0.538;P=0.006,0.030,0.039）;however,the expression level of NUCB2 mRNA in colonic tissue showed no significant difference between the two groups（t=1.835,P=0.077）.NUCB2/nesfatin-1 protein was localized in the lower 2/3 of gastric mucosal glands,Brunner＇s glands and Paneth cells of the duodenum,and Paneth cells of the small intestine.The expression level of NUCB2/nesfatin-1 protein in gastric tissues was significantly increased in obese rats compared with normal controls（Z=-2.955,P=0.003）,and was positively correlated with Lee＇s index（r=0.677,P=0.008）.The expression level of NUCB2/nesfatin-1 protein in Paneth cells of the duodenum and small intestine in obese rats significantly decreased compared with normal controls（Z=-2.026,-2.648;P=0.043,0.008）,showing a significant negative correlation with Lee＇s index（r=-0.557,-0.617;P=0.031,0.014）.CONCLUSION：NUCB2/nesfatin-1 is widely expressed in gastrointestinal tissues of rats,and the expression of NUCB2 mRNA and NUCB2/nesfatin-1 protein is up-regulated in gastric tissues of obese rats,while NUCB2/nesfatin-1 protein is down-regulated in Paneth cells in obese rats.

关 键 词：NUCB2 NESFATIN-1 肥胖 胃肠道 潘氏细胞 

分 类 号：R589.2[医药卫生—内分泌]