甲状腺功能亢进性心脏病猝死者左心室心肌连接蛋白43的分布改变  

Changes of connexin 43 distribution in myocardial cell of the left ventricle in the cases of sudden-death due to hyperthyroid heart disease

作　　者：苏波[1] 云鹏[2] 

机构地区：[1]长江大学医学院病理学教研室,湖北荆州434000 [2]长江大学临床医学院内科学教研室,湖北荆州434000

出　　处：《南京医科大学学报（自然科学版）》2013年第1期86-89,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：湖北省教育厅资助项目(XD2010185)

摘　　要：目的:探讨甲亢性心脏病猝死者心肌连接蛋白43(myocardial connexin 43,Cx43)表达及其与猝死的关系。方法:挑选长江大学医学院病理学教研室2010～2012年尸体解剖案例26例,分为2组,每组各13例。①实验组:男9例,女4例,年龄20～52岁,平均年龄34.7岁,均为甲亢性心脏病猝死。②非心源性死亡对照组:男7例,女6例,年龄22～60岁,平均年龄38.8岁。每例心脏进行常规病理学检查并取材后,运用免疫组织化学和图像分析技术,检测左心室肌的Cx43染色情况。结果:实验组心肌Cx43阳性颗粒数分布于心室肌细胞内或细胞侧-侧连接处;对照组心肌Cx43阳性颗粒数分布于闰盘。定量检测并经统计分析发现,实验组Cx43蛋白染色阳性的面积和与对照组均有非常显著的差异(P<0.01),而平均光密度比较,两组之间差异无统计学意义(P>0.05)。结论:甲亢性心脏病猝死者心室肌Cx43蛋白的分布发生明显改变,甲亢性心脏病的猝死可能与Cx43的分布变化有一定的关系。Objective：To investigate the relationship between the expression of myocardial connexin 43（Cx43）in myocardial cell of the left ventricle in the cases of sudden-death due to hyperthyroid heart disease.Methods：Selected 26 autopsy cases from the Department of Forensic Medicine School of medicine,Yangtze University during 2010 to 2012.The cases were divided into two groups,13 cases in each group.①Experimental group：9 cases male,4 female;age range is 20 to 52 with average age is 34.7 years old,Suddenly died of hyperthyroid heart disease and sudden death.② Control group：7 males,6 females,age range is 22-60 with average age is 38.8 years old.After conventional pathological examination,Cx43 in the left ventricular muscle of each case was detected with immunohistochemic staining and image analysis.Results：In control group,the Cx43 positive staining were found in the intercalated disc.In experimental group,the Cx43 positive staining were found in the cytoplasm or side-side connection of ventricular myocyte,and the positive area of Cx43 positive particles were significantly decreased than control group（P 0.01）.Conclusion：The expression of myocardial connexin（Cx43）was significantly decreased in sudden-death patients with hyperthyroid heart disease,which suggests the decreased expression of Cx43 may have relation with sudden death of hyperthyroid heart disease.

关 键 词：连接蛋白43 甲亢性心脏病 免疫组织化学 法医病理学 

分 类 号：R541.85[医药卫生—心血管疾病]