慈溪市2010-2011年手足口病病原学检测分析  

Analysis and pathogenic diagnosis of the hand-foot-and-mouth disease in Cixi during 2010 to 2011

作　　者：岑迪[1,2] 陈国华[2] 吴建根[2] 叶建杰[2] 罗莹[2] 陈坤[1] 许国章[3] 

机构地区：[1]浙江大学公共卫生学院,浙江杭州310058 [2]浙江省慈溪市疾病预防控制中心,浙江慈溪315300 [3]浙江省宁波市疾病预防控制中心,浙江宁波315010

出　　处：《中国农村卫生事业管理》2013年第1期50-54,共5页Chinese Rural Health Service Administration

基　　金：浙江省卫生厅医药卫生科学研究基金资助项目(2009A190);宁波市科技局资助项目(2009C50008)

摘　　要：目的:通过对2010-2011年手足口病(HFMD)病原学检测结果的分析,了解慈溪市HFMD流行特征。方法:收集监测哨点医院上送的临床诊断为HFMD病例标本,应用实时荧光(Real-time)RT-PCR法检测标本中的人肠道病毒(HEV)、肠道病毒71型(EV71)和柯萨奇病毒A组16型(CVA16)特异性核酸。结果:443例标本中检出总肠道病毒阳性病例394例(88.94%),其中CVA16占总阳性率的22.34%,EV71占总阳性率的56.09%。病例年龄集中在1～5岁;男女性阳性比例为1.51∶1;住院病例EV71阳性率为68.97%。结论:CVA16和EV71为该地手足口病的主要病原体,EV71是引起重症和住院病例的优势毒株型,在局部区域内CVA16型占主导。Objective To analyze the pathogenic diagnosis of the hand,foot and mouth diseases(HFMD) in Cixi during 2010 to 2011,and to understand the HFMD epidemic characteristics.Methods We collected clinically diagnosed HFMD case specimens at monitoring hospitals,using real-time fluorescence(Real-time) RT-PCR assay to test human enteric viruses(HEV),enterovirus 71 and Coxsackie virus group A type 16(CVA16) of specific nucleic acid.Results The positive rate of total intestinal virus specimen was 88.94% in 443 cases,of which Enterovirus 71 and CoxsackieA16 accounted for 56.09% and 22.34%,respectively.High positive rate was found in children aged 1-to 5-year-old.The positive rate of males and females was in the ratio of 1.51:1.The EV71-positive rate was 68.97% among inpatients.Conclusions EV71 and CVA16 are the major pathogens of HFMD in Cixi.EV71 infection is much likely to cause severe cases and hospitalization,and in some local regions CVA16 is the dominant species.

关 键 词：手足口病 病原学 肠道病毒71型(EV71) 柯萨奇A16(CA16) 实时荧光RT—PCR 

分 类 号：R725.1[医药卫生—儿科]