基于偏振滤波图像增强和动态散斑照明的宽场荧光层析显微镜  

A nnovel wide field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination

作　　者：文侨[1] 王凯歌[1] 邵永红[1] 屈军乐[1] 牛憨笨[1] 

机构地区：[1]深圳大学光电工程学院,光电子器件与系统(教育部,广东省)重点实验室,深圳518060

出　　处：《物理学报》2013年第3期143-148,共6页Acta Physica Sinica

基　　金：国家自然科学基金(批准号:61108026;61078067);高等学校博士学科点专项科研基金(批准号:20114408120001);广东高校优秀青年创新人才培养计划(批准号:LYM11107);深圳大学科研基金(批准号:201033)资助~~

摘　　要：提出了一种基于偏振滤波图像增强和动态散斑照明的新型宽场荧光层析显微镜.该显微镜采用了一种新型的偏振滤波图像增强技术,基于激发光与荧光偏振态的差异,利用偏振器件滤除激发光;并利用动态散斑照明实现宽场层析.该荧光层析显微镜具有结构简单、低成本、响应速度快、容易操作等特点.实验研究结果表明,本文提出的滤波方案能够显著地提高图像质量,利用动态散斑照明实现宽场层析具有较高的纵向分辨能力.研究丰富了在荧光显微镜中,从强激发光中提取弱荧光信号的技术手段,为今后发展具有快速响应,波长可调谐的多光谱荧光层析等高端的显微镜具有重要参考意义.We propose a novel wide-field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination in this article. A new type of polarization filtering image enhancement technology, based on the differences in polarization characteristic between excitation light and fluorescence, is used to filter out the excitation light and enhance image quality. The dynamic speckle illumination is employed to achieve the wide field fluorescence sectioning which has many advantages, such as simple configuration, low cost, rapid response and easy operation. The experimental results indicate that our filtering method can be utilized to improve the image quality significantly, and the dynamic using speckle illumination can be employed to achieve optical sectioning with a wide field and a high vertical resolution capability. Our study is not only to enrich the technological method to extract the weak fluorescence signal from the strong excitation light, but also to provide a significant reference for developing a light wavelength tunable multi-spectral fluorescence microscope with rapid response.

关 键 词：激光散斑 偏振滤波 荧光显微镜 层析 

分 类 号：O435.2[机械工程—光学工程]