HPLC法测定人血浆中百草枯的血药浓度  

Determination of paraquat in human plasma by HPLC

作　　者：叶仕远[1] 叶雪梅[1] 王陈翔[1] 周子晔[1] 胡卢丰[1] 张秀华[1] 

机构地区：[1]温州医学院附属第一医院药学部,浙江温州325000

出　　处：《中国卫生检验杂志》2013年第1期46-48,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立高效、灵敏、快速的人血浆中百草枯的HPLC血药浓度测定方法。方法:以5-溴脲嘧啶为内标物,血浆经10%三氯乙酸甲醇去蛋白。采用XBridge-C18柱,流动相为乙腈-缓冲盐(V∶V,5∶95),缓冲盐为25.0 mmol/L磷酸二氢钠,含1.0 mmol/L十二烷基硫酸钠,pH 2.6,流速1.0 ml/min,检测波长258 nm,柱温35℃。结果:百草枯和内标的出峰时间分别为6.0 min和3.5 min,百草枯在0.05μg/ml～10μg/ml范围内线性关系良好(R2=0.9998)。低、中、高3种浓度的日内、日间精密度RSD均<6%,相对回收率100.623±5.443%,平均绝对回收率88.374±5.669%。结论:本方法操作简便易行,准确率高,可作为快速、准确测定人血浆中百草枯血药浓度的有效方法,为临床诊断提供依据。Objective：To establish a rapid and effective HPLC method for determination of paraquat in human plasma.Methods： The analytes in plasma were extracted by protein precipitation using 10% trichloroacetic acid and methanol（1∶ 1）,with 5-bromine urea pyrimidine as internal standard,and separated at XBridge-C18.The mobile phase was acetonitrile-buffer solution（V∶ V,5∶ 95） that contained 25.0 mmol/L sodium dihydrogen phosphate and 1.0 mmol/L sodium dodecyl sulfate,pH was adjusted to 2.6,the flow rate was 1.0 ml/min,the UV detection wavelength was 258 nm,column temperature was 35℃.Results： The retention time of paraquat and internal standard was 6.0 min and 3.5 min.The calibration curves were linear in the range of 0.05 μg/ml～10 μg/ml（R2=0.9998）.The intra and inter-day variations were less than 6%,relative recovery and absolute recovery were 100.623±5.443% and 88.374 ±5.669%.Conclusion： The established method is simple,sensitive and accurate for determining paraquat level in human plasma.

关 键 词：百草枯 高效液相色谱法 人血浆 

分 类 号：O657.72[理学—分析化学]