甘肃人群人类血小板抗原HPA-3多态性调查  

The investigation of gene frequency of human platelet antigen-3 system in Gansu

作　　者：李国英[1] 刘圆圆[1] 薛双林 王明禄[1] 李元荣[1] 张海红[1] 方丽华[1] 唐朝晖[1] 

机构地区：[1]甘肃省血液中心,兰州730046

出　　处：《中国卫生检验杂志》2013年第1期211-213,共3页Chinese Journal of Health Laboratory Technology

基　　金：甘肃省卫生行业科研计划项目(201028)

摘　　要：目的:研究甘肃人群人类血小板抗原HPA-3系统多态性,评估其在血小板配型输注中的作用。方法:采用PCR-SSP技术对201名健康无血缘关系的血小板捐献者进行HPA-3系统基因分型。结果:HPA-3a和3b基因频率分别为0.6368和0.3632;同国内其它9个地区人群的HPA-3分布相比较,与5个地区人群有显著性差异。在随机输血中HPA-3a和3b抗原不配合的机会分别为0.1145和0.2411,a、b抗原不配合率合计为0.3556。结论:调查数据表明,随机输血中供受者HPA-3抗原不配合比例在甘肃人群中高达35.56%,这对研究血小板同种免疫性疾病和开展相合血小板输注具有指导意义。Objective：To study the polymorphism of the human platelet antigen（HPA-3） system in Gansu population,and evaluate its role in matched platelet transfusion.Methods： Two hundred and one blood samples selected from 201 unrelated platelet donors of Gansu blood center were genotyped for HPA-3 system using PCR-SSP assay.Results： The distribution of the HPA-3 alleles were genetic polymorphisms.Gene frequency of 3a and 3b were 0.6368 and 0.3632.In comparison to other 9 ethnic groups,significant differences were observed in the distribution of HPA-3 in 5 groups.The chances of incompatibility in random platelet transfusion were 0.1145 and 0.2411 for HPA-3a and-3b antigens,total 3a and 3b was 0.3556.Conclusion： The distribution of HPA-3 systems in Gansu population were investigated for the first time.The present data indicate that the chance of HPA-3 antigen incompatibility in random platelet transfusion is up to 35.56%.This study provides a basis for further study on matched transfusion of platelet.

关 键 词：血小板特异性抗原 HPA-3 输血 基因频率 甘肃汉族 

分 类 号：R331.143[医药卫生—人体生理学]