逆转录病毒介导的shRNA对大鼠原代肝细PPARα基因表达的抑制  

Inhibition effect of retroviral-mediated shRNA on expression of PPARα gene in rat primary hepatocytes

作　　者：刘辉[1] 王力[2] 

机构地区：[1]蚌埠医学院生物科学系,安徽233030 [2]蚌埠医学院预防医学系,安徽233030

出　　处：《齐齐哈尔医学院学报》2013年第1期1-2,共2页Journal of Qiqihar Medical University

基　　金：安徽省高校优秀青年人才基金(项目号:2012SQRL091)资助

摘　　要：目的包装能够干扰大鼠氧化物酶增殖物激活受体α(peroxisome proliferater activated receptorα,PPARα)基因表达的逆转录病毒,探索该病毒体外侵染SD大鼠原代肝细胞后,PPARα基因及其下游基因表达的变化情况。方法包装逆转录病毒,侵染SD大鼠原代肝细胞后抽提总RNA,荧光定量PCR检测PPARα基因及其下游基因cte1的表达。结果 PPARα基因及其下游基因cte1的表达均被抑制。结论逆转录病毒载体介导的shRNA能够有效地干扰大鼠原代肝细胞的PPARα基因的转录。Objective Retrovirus knocking down the expression of PPARα （peroxisome proliferater activated receptor） gene of rat （Rattus norvegicus ） was packaged; and the mRNA expression variations of the PPARot and its downstream gene were investigated. Methods Rat primary hepatocytes were infected by the retrovirus, and total RNA was extracted by Trizol reagent, the expression of PPARα and the downstream gene ctelwas assayed by real time PCR. Results The expression of PPARα and the downstream gene ctel were knockdown. Conclusions PPARα targeted retroviral -mediated shRNA can effectively knock down the expression of PPARα and the downstream gene ctel.

关 键 词：逆转录病毒 病毒包装 原代肝细胞 PPARΑ RNAI 

分 类 号：R99[医药卫生—毒理学]