儿童紫癜性肾炎肿瘤坏死因子α基因多态性分析  

Association of tumor necrosis factor-alpha gene polymorphisms with Henoch-Schnlein purpura nephritis in children

作　　者：王建军[1] 史艳平[2] 黄越[1] 吴春[1] 李旭昌[3] 

机构地区：[1]川北医学院附属医院儿科,四川南充637000 [2]西安市儿童医院,陕西西安710003 [3]遂宁市中心医院,四川遂宁629000

出　　处：《中国当代儿科杂志》2013年第2期88-90,共3页Chinese Journal of Contemporary Pediatrics

摘　　要：目的探讨肿瘤坏死因子α(TNF-α)-308G/A基因多态性与儿童紫癜性肾炎(HSPN)的关系。方法应用聚合酶链反应(PCR)扩增产物直接测序方法对110例过敏性紫癜(HSP组)汉族儿童进行TNF-α-308G/A基因型分析,包括紫癜性肾炎52例(HSPN组),无合并肾炎58例(单纯HSP组)。酶联免疫吸附法检测血浆TNF-α水平。90例健康汉族儿童作为对照组。结果 HSP组TNF-α-308基因型分布频率与对照组比较差异无统计学意义(P>0.05)。HSPN组GA基因型(29%)、A等位基因频率(18%)均明显高于单纯HSP组(分别为10%和7%,P<0.05),其中GA+AA基因型患儿血浆TNF-α水平(7.1±2.3 pg/mL)较GG基因型患儿(5.7±1.5 pg/mL)明显升高(P<0.05)。结论 TNF-α-308G/A基因多态性与HSP儿童肾脏损害的发生有关,A等位基因可能是儿童HSPN的易感基因。Objective To study the relationship of tumor necrosis factor-alpha （TNF-a）-308G/A gene polymorphisms with Henoch-Schonlein purpura nephritis （HSPN） in children. Methods Using the direct DNA sequencing method, polymorphisms in the TNF-et promoter region （-308） were genotyped in 110 Han children with Henoch-Schonlein purpura （HSP group） , including 52 children with nephritis and 58 children without nephritis. Plasma TNF-a levels were measured using ELISA. Ninety ethnically matched healthy children were used as the control group. Results There were no significant differences in the polymorphisms of TNF-a （-308G/A） between the HSP and control groups （P 〉0.05）. The GA genotype （29% vs 10% ） and A allele frequency （18% vs 7% ） in HSP children with nephritis （HSPN） were more common than in those without nephritis （ P 〈 0.05 ）. Plasma TNF-a levels in HSPN children with GA ＋ AA genotype （7.1 ±2.3 pg/mL） were significantly higher than those with GG genotype （5.7 ± 1.5 pg/mL） （P 〈0.05 ）. Conclusions TNF-a-308GA genotype and A allele may contribute to the increased risk for the development of nephritis in children with HSP.

关 键 词：肿瘤坏死因子Α 基因多态性 紫癜性肾炎 儿童 

分 类 号：R726.99[医药卫生—儿科]