“黄海1号”中国对虾抗白斑病的SRAP分子标记  

SRAP Molecular Marker in the WSSV Resistance of Fenneropenaeus chinensis Named "Huanghai No.1"

作　　者：张铎[1] 李梦阳[2] 杨凯[3] 赵宇亮[3] 赵宝华[3] 

机构地区：[1]河北化工医药职业技术学院制药工程系,河北石家庄050026 [2]河北衡水中学,河北衡水053000 [3]河北师范大学生命科学学院,河北石家庄050024

出　　处：《河北师范大学学报（自然科学版）》2013年第1期71-75,共5页Journal of Hebei Normal University：Natural Science

基　　金：河北省重大科技攻关项目(0760621234)

摘　　要：通过SRAP(sequence related amplified polymorphism)分子标记技术,筛选中国对虾抗对虾白斑综合症病毒(white spot syndrome virus,WSSV)的特异性序列.以中国对虾为对象,将WSSV感病组(人工投喂感染)和抗病组,使用筛选后扩增多态性良好的52对引物对其进行SRAP,并应用生物信息学方法对结果进行分析,共找到11条特异性序列可能与中国对虾WSSV抗性相关.将特异性序列在NCBI公共数据库上进行对比发现:编号为40.3,4.20和25.14的标记序列,分别跟参与表达大西洋鲑的重金属外排蛋白和表达绵羊的免疫球蛋白以及表达斑点叉尾的醛脱氢酶基因的部分序列同源性为93%,100%,87%.标记的特异性序列参与中国对虾表达相应的功能蛋白,不但可以间接提高对WSSV的抗性,而且能作为筛选中国对虾抗WSSV的分子标记进行苗种选育.The SRAP（sequence related amplified polymorphism） molecular marker was used to screen the specific sequence of WSSV（white spot syndrome virus） resistance in Fenneropenaeus chinensis.The research object F.chinensis was divided into WSSV susceptible group and WSSV resistant group,and SRAP was performed with 52 pairs of primers with good amplified polymorphism.After analyzing the results with bioinformatic methods,11 specific sequences were considered to be possilly related to WSSV resistance in F.chinensis.Then the specific sequences were compared with sequences from NCBI.The comparison showed that the marked sequences of number 40.3,4.20,25.14 had 93 %,100 %,87 % sequence identity with EST of salmon（Salmo salar） heavy metal efflux system protein,EST of sheep（Ovis aries） immunoglobulin,and EST of channel catfish（Ictalrus punctatus） aldehyde dehydrogenase,respectively.The marked specific sequences were deduced to participate the expression of corresponding functional proteins,which may not only enhance the WSSV resistance in F.chinensis indirectly,but also be used as molecular markers for WSSV resistance screen in F.chinensis breed selection.

关 键 词：对虾白斑综合症病毒 SRAP分子标记 苗种选育 

分 类 号：S336[农业科学—作物遗传育种]