不同方法对吗啡戒断大鼠脑区多巴胺系统具体影响机制的研究  

Effect of Different Methods on Brain Dopamine System of Morphine Withdrawal Rats

作　　者：卫哲[1] 董哲 尚颖[1] 宫晶[1] 

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [2]哈尔滨市儿童医院,黑龙江哈尔滨150010

出　　处：《针灸临床杂志》2013年第1期57-59,共3页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省青年科学基金项目;编号:QC2011C040;哈尔滨市科技局青年创新人才项目;编号:2012RFQXS052;黑龙江中医药大学校基金项目;编号:201013;黑龙江中医药大学创新人才支持计划"优秀青年教师支持人选"(2012)

摘　　要：目的:观察不同方法对吗啡成瘾后戒断大鼠戒断症状及NAc脑区多巴胺递质含量及其受体表达的具体影响机制。方法:采用逐日背部皮下注射盐酸吗啡注射液,末次注射后3 h给予纳络酮快速戒断,建立吗啡戒断SD大鼠模型。将50只大鼠随机分为对照组、模型组、电针组、拮抗组、针拮组,采取不同的方法治疗6天后,以ELISA法、原位杂交法分别检测NAc脑区DA含量及DA-D2RmRNA的表达。结果:与模型组比较,电针组能降低吗啡戒断后大鼠NAc脑区的DA含量(P<0.05);与模型组比较,电针组能调整戒断后大鼠NAc脑区DA-D2RmRNA的表达(P<0.05)。结论:电针可以改善吗啡戒断后症状,推测其在多巴胺系统通路中起到了类似于多巴胺受体激动剂的作用。Objective：To observe the rats＇ withdrawal symptoms,the specific influence mechanism of dopamine content in NAc brain area and the expression of dopamine transmitter after morphine addiction. Methods：Establish a morphine dependence SD rat mode.We adopted hypodermic injection by morphine in back day by day,and injected naloxone in order to rapid withdrawal after the last injection three hours.ELISA and situ hybridization were used to observe the content of DA and the expression of DA-D2RmRNA in NAc brain area. Results：Electro-acupuncture could decrease SD rats＇ DA content in Nac obviously after morphine withdrawal（P0.05）.Electro-acupuncture could adjust the expression of DA-D2RmRNA in NAc brain area after withdrawal（P0.05）.Conclusion：Electro-acupuncture can ameliorate rats＇ symptoms after morphine withdrawal.We infer that Electro-acupuncture plays the role of dopamine transmitter excitomotor similarly in dopamine system.

关 键 词：电针 吗啡 戒断 多巴胺 

分 类 号：R245.97[医药卫生—针灸推拿学]