根皮苷对2型糖尿病db／db模型小鼠主动脉的保护作用  

Effects of phlorizin on aorta of diabetes db/db mice

作　　者：沈琳[1] 高海青[1] 李保应[1] 于飞[1] 由倍安[1] 张珍[1] 

机构地区：[1]山东大学齐鲁医院老年病科山东省心血管病蛋白质组学重点实验室,济南250012

出　　处：《中华老年医学杂志》2013年第2期204-207,共4页Chinese Journal of Geriatrics

基　　金：山东省医药卫生科技发展计划（2011HZ048）

摘　　要：目的观察根皮苷对2型糖尿病小鼠主动脉的保护作用，探讨其可能的作用机制。方法以db／m小鼠8只作为正常对照组（对照组），16只糖尿病db／db小鼠随机分为2组：糖尿病组和糖尿病干预组。干预组给根皮苷（20mg／kg）盐水灌胃10周。对照组和糖尿病组小鼠每天用相同体积的生理盐水灌胃10周。所有小鼠每周称体质量。10周末空腹处死全部小鼠，留取血清和主动脉。检测血清空腹血糖、三酰甘油、总胆固醇、糖基化终产物和丙二醛浓度。主动脉做病理学检查。结果10周末，糖尿病组体质量（57．53±3．40）g、空腹血糖（31．21±2．16）mmol／L、三酰甘油（0．39±0．12）mmol／L，总胆固醇（3．15±0．30）mmol／L、糖基化终产物（0．28±0．04）AU／mg和丙二醛（15．18±1．60）mol／L水平高于对照组（P〈O．01）。干预组体质量（54．24±1．28）g、血清空腹血糖（29．17±1．41）mmol／L、三酰甘油（0．26±0．06）mmol／L、总胆固醇（2．71±0．26）mmol／L、糖基化终产物（O．24±0．03）AU／mg和丙二醛（13．46±1．28）nmol／L水平较糖尿病组降低（P〈0．05或P〈0．01）。干预组主动脉病变较糖尿病组减轻。结论根皮苷对糖尿病小鼠的大血管具有保护作用，该作用与其降糖调脂、降低糖基化终产物水平和抗氧化有关。Objective To observe the effects of phlorizin on aorta of diabetes db/db mice and to explore its mechanism. Methods Sixteen diabetes db/db mice were randomized into two groups： untreated diabetic group （DM group） and diabetic group treated by phlorizin（DMT group） with eight db/m mice as normal control group（CC group）. Phlorizin （20 mg/kg） was given in normal saline solution intragastrically to the mice of the DMT group for 10 weeks. Mice of the other two groups were given the same amount of saline solution intragastrically for 10 weeks. Animals were weighed weekly. At 10th weekend, all mice were fasted overnight and then sacrificed. Fasting blood was collected, and aortas were dissected. The blood samples were analyzed for fasting blood glucose （FBG） ,triglycerides （TG）, total cholesterol （TC）, Serum advanced glycation end products （AGEs） and malondialdehyde （MDA）. Aortic tissue were examined microscopically. Results At 10th weekend, the weight （57.53±3.40）g and serum concentration of FBG（31. 21±2.16） mmol/I.,TG （0.39±0. 12） mmol/L,TC（3. 15±0.30 ）mmol/L,AGEs （0.28±0.04） AU/mg and MDA （15. 18±1.60）mol/I, in DM group were increased than those in CC group （P〈0.01）, and the weight（54.24± 1.28）g,FBG（29.17±1.41） mmol/L,TG（0.26±0.06）mmol/L,TC（2.71±0.26） mmol/I.,AGEs （0.24±0.03） AU/mg and MDA（13.46±1. 28）mol/L were lowered significantly in DMT group than those in DM group （P〈0.05 or P〈0.01）. The severity of aorta damage in DMT group was less than that in DM group. Conclusions Phlorizin can protect the db/db mice from diabetic macrovascular complications, which may be attributed to its decreasing of blood glucose, TG, TC, and AGEs levels, and its antioxidant potential.

关 键 词：糖尿病 2型 根皮苷 主动脉 氧化性应激 

分 类 号：R285.5[医药卫生—中药学]