补肾解毒方与健脾解毒方含药血浆对慢性乙肝病毒感染不同免疫状态患者外周血树突状细胞功能的影响  

Effects of Bushen Jiedu Recipe and Jianpi Jiedu Recipe Containing Plasma on Dendritic Cells of Chronic Hepatitis B Virus Infection Patients under Different Immune States

作　　者：欧松[1] 孙克伟[2] 彭建平[2] 祁双林[1] 闻杰[1] 胡莉[2] 

机构地区：[1]湖南中医药大学中西医结合学院,长沙410007 [2]湖南中医药大学第一附属医院感染科,长沙410007

出　　处：《中国中西医结合杂志》2013年第2期208-213,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81072779);湖南省自然科学基金项目(No.09JJ6042)

摘　　要：目的比较补肾解毒方和健脾解毒方对慢性乙肝病毒(HBV)感染不同免疫状态患者外周血树突状细胞(DCs)功能的影响。方法选择36例湖南中医药大学第一附属医院2010年4月—2011年1月门诊慢性HBV感染患者,根据患者免疫状态分为免疫耐受组(18例)、免疫清除组(18例),另选择10名健康人作健康组。分别采集抗凝外周静脉血,分离外周血单个核细胞(PBMCs),进一步分离提取培养DCs,采用大鼠补肾解毒方和健脾解毒方含药血浆进行干预。对DCs进行形态学鉴定,并检测DCs表型CD1α、CD80、CD86、HLA-DR表达,观察DCs培养上清液干扰素-α(interferon-α,IFN-α)的水平。结果免疫清除组患者干预前CD80低于健康组(P<0.05);免疫耐受组患者干预前CD80、CD86、HLA-DR均低于健康组;免疫耐受组与免疫清除组干预前IFN-α低于健康组(P<0.05),免疫耐受组患者干预前CD80、HLA-DR、IFN-α低于免疫清除组(P<0.05)。与本组干预前比较,各给药组CD80表达均升高(P<0.05)。免疫耐受组患者干预后CD80、HLA-DR高于免疫清除组同期,补肾解毒方组CD86高于免疫清除组同期,差异有统计学意义(P<0.05)。结论补肾解毒方中剂量和健脾解毒方小剂量均能促进慢性HBV感染患者DCs功能的恢复,且补肾解毒方对慢性HBV感染免疫耐受期患者DCs功能的影响更为显著。ObjectiveTo compare the effects of Bushen Jiedu Recipe （BJR） and Jianpi Jiedu Recipe （JJR） containing plasma on dendritic cells （DCs） of chronic hepatitis B virus （HBV） infection patients under different immune states. MethodsRecruited were 36 chronic HBV infection outpatients from First Affiliated Hospital of Hunan University of Traditional Chinese Medicine from April 2010 to January 2011. They were assigned to the immune tolerance group （18 cases） and the immune clearance group （18 cases）. Another 10 healthy subjects were recruited as the healthy control group. Their anticoagulated peripheral venous blood was respectively collected. The peripheral blood mononuclear cells （PBMCs） were isolated and further extracted for incubating DCs. The DCs were intervened by BJR and JJR containing plasma. The morphology of DCs was identified. The expressions of CD1α， CD80， CD86， and HLA-DR were detected. The level of interferon-α （IFN-α） in the supernatant was observed by ELISA. ResultsThe CD80 expression level was lower in the immune clear group than in the healthy control group before intervention （P〈0.05）. The expression levels of CD80， CD86， and HLA-DR were lower in the immune tolerance group than in the healthy control group before intervention （P〈0.05）. The IFN-α expression level was lower in the immune tolerance group and the immune clearance group than in the healthy control group before intervention （P〈0.05）. The expression levels of CD80， HLA-DR， and IFN-α were lower in the immune tolerance group than in the immune clearance group before intervention （P〈0.05）. Compared with the same group before intervention， the CD80 expression significantly increased in each treatment group （P〈0.05）. After intervention the expression levels of CD80 and HLA-DR were higher in the immune tolerance group than in the immune clearance group in the same time phase， and the CD86 expression level was higher in the BJR group than in the immune clearance g

关 键 词：乙肝病毒感染 补肾解毒方 健脾解毒方 外周血 树突状细胞 

分 类 号：R512.62[医药卫生—内科学]