正清风痛宁片联合甲氨蝶呤对胶原诱导的关节炎大鼠血清OPG/RANKL、IL-17的影响  

Effects of Zhengqing Fengtongning Tablet and Methotrexate on the Serum OPG/RANKL and IL-17 of Collagen-induced Arthritis Rats

作　　者：丁从珠[1] 姚瑶[2] 方芸[2] 孙凌云[2] 汪悦[3] 

机构地区：[1]南京中医药大学中西结合鼓楼临床医学院风湿免疫科,南京210008 [2]南京中医药大学中西结合鼓楼临床医学院药学部,南京210008 [3]南京中医药大学第一临床医学院,南京210029

出　　处：《中国中西医结合杂志》2013年第2期256-260,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：南京市医学重点科技发展项目(No.ZKX08022);江苏省"六大高峰"人才资助项目(No.2010-057);江苏省研究生创新项目(No.762);南京市医学科技发展项目(No.YKK11101)

摘　　要：目的探讨正清风痛宁片联合甲氨蝶呤(MTX)对胶原诱导的关节炎(CIA)大鼠血清骨保护素(OPG)、核周因子κB受体活化因子配体(RANKL)、白细胞介素17(IL-17)的作用,了解其联合治疗的骨保护作用。方法于大鼠背部皮内、尾根注射Ⅱ型胶原乳剂建立CIA大鼠模型,选取造模成功且关节炎指数(AI)>2分的大鼠28只,随机分为模型组、中药组、MTX组、联合组,每组7只,另选取7只作为正常对照组。造模后7天开始灌胃给药,MTX组予MTX(3.8mg/kg)每周1次;中药组予正清风痛宁片[130mg/(kg·d)];联合组予正清风痛宁片[130mg/(kg·d)]和MTX(3.8mg/kg)每周1次;模型组与正常对照组予等体积生理盐水灌胃,各组均干预26天。采用X线摄片观察大鼠四肢关节破坏情况,记录大鼠AI评分,实验结束后检测各组大鼠血清OPG、RANKL、IL-17表达水平。结果实验过程中,除模型组外,其余大鼠状态良好。造模第21天,各组大鼠AI评分均达到峰值,干预后各组AI评分均降低,与模型组比较,干预第26天中药组、MTX组、联合组AI评分降低,差异有统计学意义(P<0.05)。与模型组比较,MTX组干预后OPG、RANKL降低,中药组OPG升高,OPG/RANKL升高,联合组OPG、RANKL及OPG/RANKL升高,IL-17降低,差异均有统计学意义(P<0.05)。与MTX及中药组比较,联合组OPG/RANKL升高(P<0.05),IL-17降低(P<0.05)。结论正清风痛宁片联合MTX能够协同提高CIA模型大鼠外周血OPG/RANKL,下调IL-17。Objective To study the effects of Zhengqing Fengtongning Tablet （ZFT） and methotrexate （MTX） on the expression of osteoprotegerin （OPG）， receptor activator of nuclear factor-κB ligand （RANKL）， and interleukin 17 （IL-17） in collagen-induced arthritis （CIA） rats， thus addressing their bone protection. Methods The CIA rat model was established by intradermally injecting type Ⅱ collagen emulsion from the rats′ back and tail. Totally 28 successfully modeled rats ［with the arthritis index （AI） more than 2］ were randomly divided into the model group， the Chinese medicine （CM） treatment group， the MTX group， and the ZFT＋MTX treatment group， 7 rats in each group. Another 7 rats were recruited as the normal control group. Rats were administered from the 7th day of modeling. Rats in the MTX group were treated with MTX at 3.8 mg/kg once a week. Those in the CM group were treated with ZFT at the daily dose of 130 mg/kg， once a day. Those in the ZFT＋MTX treatment group were treated with both MTX （at 3.8 mg/kg once a week） and ZFT （at the daily dose of 130 mg/kg， once a day）. Those in the model group and the normal control group were administered with normal saline of the equal volume by gastrogavage. All the intervention lasted for 26 days. The destruction of joints in the four limbs were observed using X-ray. The AI was recorded. The expression levels of serum OPG， RANKL， and IL-17 were detected at the end of the experiment. Results During the whole process， all rats except those in the model group were in a good condition. On the 21st day of modeling the AI of all rats reached the peak， but it decreased after treatment. Compared with the model group， the AI decreased in the CM treatment group， the MTX group， and the ZFT＋MTX treatment group with statistical difference （P〈0.05）. Compared with the model group， the OPG increased and RANKL decreased in the MTX group； the OPG and OPG/RANKL increased in the CM treatment group； the OP

关 键 词：类风湿关节炎 胶原诱导的关节炎 骨保护素 核因子-κB活化因子配体 正清风痛宁片 甲氨蝶呤 

分 类 号：R684[医药卫生—骨科学]