2011年上海地区轻、重症手足口病患者肠道病毒71型分离株VP1、VP4区的基因特征分析  

Sequence analysis of VP1 and VP4 genes of enterovirus 71 strains isolated from severe and mild hanD- foot-mouth disease cases in Shanghai, China, 2011

作　　者：蔡韵[1] 申惠国[2] 李治俊[2] 居丽雯[1] 蒋露芳[1] 熊海燕[1] 姜庆五[1] 

机构地区：[1]复旦大学公共卫生学院流行病学教研室公共卫生安全教育部重点实验室,上海200032 [2]上海市闵行区疾病预防控制中心

出　　处：《中华传染病杂志》2013年第1期12-18,共7页Chinese Journal of Infectious Diseases

基　　金：十一五重大专项新发和突发传染病高通量检测技术的开发及其临床应用评估资助项目（2009ZX10004-104）;上海市重点学科建设资助项目（B118）

摘　　要：目的了解2011年上海地区手足口病重症和轻症患儿中肠道病毒71型（EV71）分离株VP1、VP4区的基因特征。方法对来自2011年重症与轻症手足口病患儿的各5株EV71分离株进行VP1、VP4全序列的RT—PCR扩增测序，并与美国国立生物技术信息中心公布的EV71A、B、C基因型代表株进行核苷酸、氨基酸比对分析和系统进化分析。结果轻、重症患儿的EV71分离株之间VP1基因的核苷酸同源性为96．0％～98．1％；VP4基因的核苷酸同源性为93．7％～99．5％。轻、重症患儿的EV71分离株与C基因型代表株比较接近，VP1区核苷酸同源性分别为86．9％～98．2％、87．4％～98．5％，VP4区核苷酸同源性分别为85．5％～100．0％、84．5％～99．5％，其中与2008年安徽省阜阳市的EV71流行株（C4亚型）VP1区核苷酸同源性分别可达97．0％～98．2％、97．9％～98．5％，VP4区核苷酸同源性分别可达96．1％～100．0％、97．1％～99．5％。3例重症患儿分离株在VP1和VP4的天冬酰胺（N）282丝氨酸（S）、苏氨酸（T）7丙氨酸（A）同时发生变异。结论2011年上海地区10例轻、重症手足口病患儿中分离的EV71流行株均属C基因型的C4亚型；3例重症患儿分离株在VP1和VP4的N282S、T7A同时发生变异。Objective To analyze the VP1 and VP4 genetic region of enterovirus 71 （EV71） isolated from severe cases and mild cases with hanD foot-mouth disease （HFMD） in Shanghai in 2011. Methods Five EV71 strains isolated from severe cases and five EVT1 strains from mild cases in 2011 were included. Reverse transcription-polymerase chain reaction （RT-PCR） method was used to amplify and sequence the VP1 and VP4 genes of EV71, and then the sequencing results were compared with those of A, B, C genotype reference EV71 strains from GenBank by nucleotide alignment, amino acid alignment and phylogenetic tree analyses. Results The homogeneity between EVT1 strains from severe cases and mild cases was 96.0%--98. 1% and 93.7%--99. 5% for VP1 and VP4 nucleotide sequences, respectively. The VP1 nucleotide sequences of 5 strains isolated from severe cases and 5 strains from mild cases in Shanghai shared 86. 9%--98. 2% and 87. 4%--98. 5% identity with genotype C, respectively, while the homogeneity of VP4 nucleotide sequence was 85. 5 %--100.0% and 84.5%- 99.5%, respectively. In addition, compared with the Fuyang EV71 strains （representative of C4 subtype）, the strains from mild and severe cases shared homogeneity of 97.0%--98.2% and 97.9%- 98.5% for VP1 gene, respectively, 96. 1%--100.0% and 97. 1%-- 99.5% for VP4 gene, respectively. Among 3 strains isolated from severe eases, mutations at the residue 282 in the VP1 protein （N→S） and residue 7 in the VP4 protein （T→A） were discovered simultaneously. Conclusions The 10 EV71 strains isolated from severe and mild cases in Shanghai belong to subgenogroup C4. Among 3 strains isolated from severe cases, mutations at the residue 282 in the VP1 protein （N→S） and residue 7 in the VP4 protein（T→A） are discovered simultaneously.

关 键 词：手足口病 肠道病毒A型 人 基因 病毒 序列分析 基因型 变异(遗传学) 

分 类 号：R72[医药卫生—儿科]