Perfadex液与UW液对猪肺动脉内皮细胞功能的影响  

Effect of Perfadex and Wisconsin Unviersity solution on the function of pulmonary artery endothelium

作　　者：冯志强[1] 李世康[1] 胡应学[1] 黄强信[1] 周琦云[1] 何立术[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《中华器官移植杂志》2013年第2期114-116,共3页Chinese Journal of Organ Transplantation

基　　金：广西医疗卫生重点研究课题（重200827）

摘　　要：目的探讨Perfadex液与UW液低温保存对猪肺动脉内皮细胞功能的影响。方法取新鲜猪肺的小叶间动脉，制备为6条2mm长的血管环，按随机数值表法分入KH液组、Perfadex液组和UW液组，每组2条血管环在相应保存液中保存4h。观察在37℃下经吲哚美辛、N-硝基-L-精氨酸和氧合血红蛋白作用后，地诺前列素氨丁三醇引发的血管收缩反应，及不同浓度的缓激肽和非受体介导钙离子载体引发的血管舒张反应。结果地诺前列素氨丁三醇引发的各组血管收缩强度的差异无统计学意义（P〉0．05）。比较各组由缓激肽及非受体介导钙离子载体引发的血管环最大舒张反应的差异，UW液组明显低于KH液组（P〈0．01），而Perfadex液组和KH液组的差异无统计学意义（P〉0．05）。结论内皮源性超极化因子（EDHF）在猪肺动脉内皮依赖性舒张过程中起着重要的作用。UW液低温保存会损伤肺动脉EDHF所介导的内皮依赖性舒张功能，而Perfadex液对其无明显影响。Objective To study the effect of Perfadex and Wisconsin Unviersity solution on the function of pulmonary artery endotheliurn. Methods Small lobe pulmonary arteries were dissected from nine porcine lungs. The artery from each lung was cut into six rings in 2mm. Two of them were randomly incubated in Krebs, Perfadex or Wisconsin Unviersity solution （UW solution） at 4℃ for 4 hours. Endothelium-derived hyperpolarizing factor （EDHF）-mediated relaxation （percentage of -7.5 logM U46619 precontraction） induced by bradykinin or calcium ionophone A23187 in the present of indomethacin, L-NNA and oxyhemoglobin were measured at 37 ℃ in the organ chambers. Results Vasoconstriction induced by U46619 is no significant difference among three groups （P〉 0. 05）. Compared with Krebs group, the relaxation induced by bradykinin or A23187 was significantly decreased in Perfadex group （P〈0. 01 ）, while there is no significant difference in UW group （P〉 0. 05）. Conclusion Endothelium derived hyperpolarizing factor （EDHF） plays an important role in endothelium-mediated relaxation of porcine pulmonary artery. EDHF-mediated relaxation is impaired when the lung preserved with Wisconsin Unviersity solution, wheras its function is not affected by Perfadex solution.

关 键 词：肺动脉 内皮源性超极化因子 Perfadex液 UW液 

分 类 号：R655.3[医药卫生—外科学]