浙江省1例输入性卵形疟的实验室检测分析  

Laboratory Testing for a Case of Imported Plasmodium ovale Infection in Zhejiang Province

作　　者：王晓光[1] 雷永良[1] 兰进权[1] 梅建华[1] 李祖火[1] 

机构地区：[1]浙江省丽水市疾病预防控制中心,丽水323000

出　　处：《中国寄生虫学与寄生虫病杂志》2013年第1期78-79,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：利用病原学和分子生物学方法,对浙江省1例输入性卵形疟原虫感染者(源自赤道几内亚)血样进行实验室确诊检测。取患者服用抗疟药物前血样50μl,涂制薄、厚血片,自然干燥、甲醇固定、吉氏染色,于光学显微镜下查找疟原虫。提取患者血样基因组DNA,实时荧光PCR检测其是否含有卵形疟、间日疟、恶性疟或三日疟原虫特异性DNA片段。镜检结果显示,患者血样薄、厚血片均发现卵形疟原虫虫体。荧光定量PCR结果显示,患者血样为卵形疟原虫特异性DNA片段阳性。依据实验室检测结果、流行病学资料和临床表现,确诊该患者为输入性卵形疟原虫感染病例。Blood sample obtained from a patient, which returned from Equatorial Guinea, with clinical diagnosis of Plasmodium infection was confirmed as imported P. ovale infection by etiology and molecular biological methods. 50 μl blood was obtained before taking anti-malarial drugs to make thin and thick blood smears, Giemsa stained, and observed by microscopy. Genomic DNA was extracted from the blood sample, and detected for DNA fragment of P. ovale, P. vi- vax, P. falciparum or P. malariae by real-time fluorescent quantitative PCR. P. ovale parasites were found in both thin and thick blood smears, and confirmed by quantitative PCR. With the results of laboratory testing, epidemiological history and clinical manifestations, the patient was diagnosed as imported P. ovale infection.

关 键 词：卵形疟 输入病例 实验室检测 实时荧光PCR 

分 类 号：R531.39[医药卫生—内科学]