外源亚精胺对盐胁迫下毕氏海蓬子体内多胺含量的影响  

Effects of Exogenous Spermidine on the Content of Polyamines in Salicornia bigelovii Torr.under NaCl Stress

作　　者：周峰[1] 华春[1] 丁春霞[1] 王仁雷[2] 李萍[1,3] 郑春梅[1,3] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,南京211171 [2]江苏教育学院,南京210013 [3]南京师范大学生命科学学院,南京210046

出　　处：《西北植物学报》2013年第1期83-89,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省自然科学基金青年基金项目(BK2012073);国家高技术研究发展计划(2012AA021701);江苏省生态学重点学科建设项目(2012);南京晓庄学院生物学重点学科建设项目(2012)

摘　　要：用含不同浓度(0、100、200、300、400和500mmol.L-1)NaCl的Hoagland营养液及叶面喷施0.1mmol.L-1外源亚精胺(Spd)处理毕氏海蓬子幼苗,研究外源Spd对NaCl胁迫下海蓬子体内游离态、结合态和束缚态3种形态多胺含量的影响,分析内源多胺含量的变化与植物耐盐性的关系。结果表明:(1)随盐胁迫浓度的升高,海蓬子幼苗叶片中3种形态腐胺(Put)含量均先降后升;同期的游离态Spd含量持续上升,结合态和束缚态Spd含量均先升后降;同期的游离和结合态精胺(Spm)含量均先升后降,而其束缚态Spm含量呈上升趋势;游离态和束缚态多胺(PAs)总量变化随盐浓度升高均呈上升趋势,而结合态PAs总量先升后降。(2)3种形态(Spd+Spm)/Put比值均先升后降,而3种形态Put/PAs比值则均呈先降后升的相反趋势。(3)外源Spd处理提高了海蓬子幼苗叶片中结合态和束缚态PAs总量,也提高了游离态和束缚态(Spd+Spm)/Put比值。研究发现,外源Spd参与了NaCl胁迫下海蓬子内源PAs代谢的调节,可能通过促进盐胁迫植株中Put向Spd和Spm的转化,以及游离态多胺向结合态和束缚态多胺的转化来增强海蓬子耐盐性。Seedlings of Salicornia bigelovii Torr.were treated with different concentrations of NaCl（0,100,200,300,400,500 mmol·L-1）.Three kind of polyamines and their different forms were investigated under NaCl and exogenous spermidine treatment.The results showed that：（1）three forms of the putrescine（Put） increased at first and then decreased under salt treatment.The free form of spermidine（Spd） and the bound state of spermine（Spm） increased all the time.The free form of Spd,the conjugated state of Spd and Spm,and the bound state of Spd both increased at first and then decreased.Finally,the total polyamines（PAs） of the free and bound were both increased all the time,while the conjugated state content increased at first and then decreased.（2）All the three forms of polyamines of（Spd＋Spm）/Put ratio increased at first and then decreased,while the Put/PAs ratio was decreased first and then increased.（3）The conjugated and bound form of the polyamines contents increased with exogenous Spd treatment.At the same time,the free and bound states of（Spd＋Spm）/Put ratio also increased.It indicated that exogenous Spd could be involved in the regulation of PAs metabolism and played an important role in enhancing salt tolerance of S.bigelovii seedlings.

关 键 词：毕氏海蓬子 多胺 亚精胺 

分 类 号：Q945.78[生物学—植物学]