一种新型慢性压迫性颈髓损伤大鼠模型的建立  

Pathological validation of a novel experimental chronic compressive cervical myelopathy rat model

作　　者：李广盛[1] 龙厚清[1] 林尔坚[2] 谢文翰[1] 胡勇[3] 

机构地区：[1]中山大学附属第一医院脊柱外科,广州510080 [2]中山大学附属第一医院放射科 [3]香港大学骨矫形与创伤外科

出　　处：《中华显微外科杂志》2013年第1期46-51,共6页Chinese Journal of Microsurgery

基　　金：广东省自然科学基金资助项目（S2011010004843）

摘　　要：目的建立并验证一种新型慢性压迫性颈脊髓损伤大鼠模型。方法2011年6—2011年11月，将54只SD大鼠随机分为对照组（n=6）、急性压迫组（4h、24h，n=6）、慢性压迫组（4h、12h、24h、48h、72h、1周，n=6）。在C5-6硬膜外间隙置入不同规格吸水聚氨酯胶片制作急、慢性压迫性脊髓损伤模型。造模后不同时间行MRI、组织学和组织化学观察，运动功能评分（BBB）评价神经功能。结果①急性压迫组4～24h，T2WI见脊髓明显受压、髓内高信号，组织学见脊髓出血坏死，前角神经元计数显著低于对照、慢性压迫组（P〈0．05），BBB评6．0分。②慢性压迫4—12h，见脊髓水肿、中央管变形，神经元计数、髓鞘染色强度与对照组比较，差异无统计学意义（P〉0．05），BBB评20．6分；24～72h，脊髓受压明显，中央管扩大、静脉淤血，神经元计数减少（P〈0．05），后索神经纤维排列紊乱、断裂，髓鞘染色强度低于对照组（P〈0．05），BBB评19．3分；1周，髓内空泡化，神经元数目减少（P〈0．05），后索髓鞘蓝染强度低于对照组（P〈0．05），BBB评17．5分。LWI示脊髓受压变形，髓内未见出血信号。结论慢性压迫组造模方法可以实现慢性压迫性脊髓损伤，符合慢性压迫性脊髓损伤的病理、影像学和神经功能变化特征。Objective To establish a novel chronic compressive cervical spinal coM injury rat model, to validate the chronic pathological characteristic. Methods Fifty-four SD rats were randomly divided into 3 groups, including control group（ n = 6） , acute compressive group （4 h, 24 h,respectively, n = 6） , and chronic compressive group（4 h, 12 h, 24 h, 48 h, 72 h and 1week,respectively. n =6）. Two sizes of water-absorbing polyurethane polymer sheets were implanted into C5.~ epidural space on postero-lateral side in acute and chronic compressive group respectively, to induce a consistent compression in the cord after expanded. While a laminec- tomy on C54 was performed only in control group. The neurological integrity, MRI signal change in the cords, large motoneuron number in the ventral horn, and myelin staining intensity on posterior funiculus were studied. Results In the acute group al 4-24 h, the compression was confirmed significantly on T2 WI image, as well as hypointense signal change intramedullary. These changes were consisted with intramedullary bleeding, tissue necrosis. Large motoneuron number（ P 〈 0. 05 ）, rather than myelin staining intensity （ P 〉 0. 05 ）, was signifi- cantly decreased compared with the control group and chronic compressive group. BBB score was 6. 0 at 24 h. In the chronic group： cord distortion with progressive compression was observed on To WI image, but without in- tramedullary bleeding signal change. At 4 -12 h,intramedullary edema, central canal distortion were seen in the cords. Large motoneuron number and myelin staining intensity decrement were not significant（P 〉 0. 05）. BBB score was 20. 6. At 24-72 h, central canal enlargement, venous congestion, and edema were observed. Large motoneuron number was less than that in the control group. In the compressive epicenter, nerve fiber disorgani- zation or rupture was observed. Myelin staining intensity decreased significantly after 48 h and 72 h compression （P 〈 O. 05 ）. BBB score was 19. 3. At 1 week

关 键 词：慢性压迫性脊髓损伤 脊髓型颈椎病 大鼠 模型 

分 类 号：R651[医药卫生—外科学] R-332[医药卫生—临床医学]