转AtDREB1A基因菊花杂交后代优株水分胁迫耐性分析  

Drought Stress Tolerance of the Hybrid Offspring Produced by AtDREB1A Transgenic Chrysanthemum

作　　者：杨英杰[1] 李春水[1] 张晓娇 魏倩[1] 高俊平[1] 洪波[1] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100193 [2]内蒙古鄂尔多斯东胜区园林局,鄂尔多斯017000

出　　处：《农业生物技术学报》2013年第2期148-157,共10页Journal of Agricultural Biotechnology

基　　金：北京市园林绿化局育种专项(No.YlHH2008002);国家自然基金项目(No.30972022)

摘　　要：过量表达拟南芥(Arabidopsis thaliana)逆境诱导转录因子DREB1A基因(AtDREB1A)能提高菊花的干旱胁迫耐性。以具有干旱胁迫耐性的转AtDREB1A基因菊花(Chrys anthemum morifolium)株系与广泛应用的非转基因菊花品种进行常规杂交获得的后代优株A-121、A-128、A-136为实验材料,进行了RT-PCR检测、水分胁迫下的萎蔫指数和成活率统计以及脯氨酸(Pro)含量和超氧化物歧化酶(SOD)活性测定。结果显示,外源AtDREB1A基因能够在有性繁殖过程中遗传给后代,且在水分胁迫下能在杂交后代植株中表达;与对照相比,水分胁迫条件下,转基因植株杂交后代Pro含量和SOD活性明显升高,且具有较强的水分胁迫耐性。研究结果表明,转基因菊花所携带的AtDREB1A基因可以在常规杂交中稳定遗传并发挥功能,提高了植株对水分胁迫的耐性。本研究为通过基因工程和传统育种结合方式选育具有干旱耐性的菊花新品种提供了技术平台。Overexpression of the stress-inducible transcription factor gene A tDREB1A in Chrysanthemum conferred strong drought stress tolerance. Taking A-112, A-128 and A-136 as experimental materials, which are the three excellent hybrid offspring plants of the ground cover Chrysanthemum fall color transgenic line 1805 with drought stress tolerance harboring Ar^idopsis thaliana dehydration response element-binding protein 1A （AtDREBIA） and the Asia Winter Light with better phenotype, we analyzed the stability and expression of AtDREBIA under water stress condition by RT-PCR, and determined the wilting index, survival rate, proline（Pro） content and superoxide dismutase（SOD） activity under drought stress condition. The results demonstrated that the exogenous gene A tDREBIA was transferred into the hybrid offspring plants by sexual propagation and it could be expressed in offspring plants under drought condition. Compared with the control plants, the hybrid offspring plants had obviously stronger drought stress tolerance, and their Pro content and SOD activity were obviously raised under drought condition. These results indicated that the A tDREBIA gene which is transferred into the hybrid offspring plants functions in enhancing drought stress tolerance. This research plays a role in breeding new Chrysanthemum cultivars with drought stress tolerance by genetic engineering combined with traditional hybrid breeding.

关 键 词：菊花 AtDREB1A 杂交后代 水分胁迫耐性 

分 类 号：S512.1[农业科学—作物学]