山东地方鸡MHCB-F基因遗传变异与免疫性状相关性研究  

Associations between Immune Traits and Major Histocompatibility Complex(MHC) B-F Gene in Shandong Local Chicken(Gallus domesticus) Breeds

作　　者：李福伟[1,2] 逯岩 雷秋霞[1,2] 韩海霞 周艳[1,2] 李桂明 武彬[1,2] 曹顶国 

机构地区：[1]山东省农业科学院家禽研究所,济南250023 [2]山东省家禽育种工程技术研究中心,济南250023

出　　处：《农业生物技术学报》2013年第2期179-184,共6页Journal of Agricultural Biotechnology

基　　金：山东省自然基金(No.ZR2009DM004);山东省良种工程高新育种子课题(No.2008LZ015)

摘　　要：鸡主要组织相容性复合体(MHC)由一组紧密连锁的、高度多态的基因座位所组成,在机体的免疫应答和调控等方面具有极其重要的作用。本研究旨在研究3个地方鸡(Gallus domesticus)品种(鲁禽鸡、琅琊鸡、石岐杂鸡)Major histocompatibility complex(MHC)B-F基因遗传变异与Sheep red blood cell(SRBC)、Avianinfluenza(AI)和New castle disease(ND)免疫抗体滴度等免疫性状的关系,揭示不同品种间基因变异与免疫性状的相关性。研究结果表明:3个品种SRBC免疫抗体滴度石歧杂鸡最高,与另外两个品种差异极显著(P<0.01);ND、H9抗体滴度趋势与SRBC一致,石歧杂另外两个品种差异极显著(P<0.01);H5抗体滴度石歧杂鸡最低,与另外两个品种差异极显著(P<0.01);亲缘关系较近的琅琊鸡和鲁禽鸡在4个抗体间结果基本一致,2个品种间差异均不显著(P>0.05)。通过PCR-SSCP方法分析了包括MHCB-F基因外显子2在内长度为396bp的片段。在这3个品种中分别发现了49、45和41个核苷酸变异位点,其中分别有40、37和32个引起了氨基酸突变,关联分析结果表明:3个品种中分别有6、9、4个SNPs位点与所检测的免疫性状显著相关(P<0.05)。亲缘关系最近的鲁禽和琅琊鸡其基因变异与免疫性状相关性基本一致,二者共有5个变异位点与免疫性状相关性一致,其中位点A192C、A217G均与新城疫抗体滴度显著相关(P<0.05),位点A217G与禽流感H5抗体滴度显著相关(P<0.05),位点A211G与禽流感H9抗体滴度显著相关(P<0.05),位点G232A与绵羊红细胞抗体滴度显著相关(P<0.05)。在石歧杂鸡中位点C183G与新城疫、禽流感H9抗体滴度显著相关(P<0.05)。研究结果对于临床疫苗的使用具有重要的指导作用。The chicken Major histocompatibility complex （MHC） is composed of a group of closely linked, highly polymorphic loci composition, which has an extremely important role in the body＇s immune response and regulation. This experiment was conducted to study the association between immune traits（Sheep red blood cell（SRBC）, Avian influenza（AI） and Newcastle disease（ND）） and Major histocompatibility complex（MHC） B-F gene in Shandong local chickens （Gallus domesticus） breeds （Luqin chicken, Shiqiza chicken and Langya chicken）, The results of comparison ofAI, ND and SRBC titers among the three breeds showed that the antibody titers of SRBC, ND, and H9 in Shiqiza chicken were significantly higher than the two breeds （P〈0.01）; but the antibody titers of H5 in Shiqiza chicken were significantly lower than the two breeds （P〈0.01）; The Luqin chicken had the closest genetic relationship to Langya chicken, the antibody titers of them were basically the same and no significant difference with the other two populations（P〉0.05）. A 396 bp sequence including exon 2 of chicken MHC B-Fgene was assessed in this study by the PCR-SSCP. The results indicated that 49, 45 and 41 SNPs were respectively detected in Luqin chicken, Shiqiza chicken and Langya chicken. Among them, 40, 37 and 32 nucleotide variations affected amino acid variations. Based on the three varieties, six, nine and four SNPs were respectively found significantly effect on partial immune traits（P〈0.05）. The Luqin chicken had the closest genetic relationship to Langya chicken, the genetic variation of MHC B-F and antibody titers of them were basically the same. Both in Luqin chicken and Langya chicken, a total of 5 identical variants were found significant correlation with immune traits. The loci G232A and A217G were significantly associated with Newcastle disease antibody titers （P〈0.05） and the A217G was significantly associated with avian influenza H5 antibody titers （P〈0.05）. Locus A211G was significan

关 键 词：鸡 MHC B—F PCR—SSCP 免疫性状 

分 类 号：S852.4[农业科学—基础兽医学]