马铃薯黑痣病菌AG2-1和AG3融合群分析  

Analysis on Anastomosis Groups of Rhizoctonia solani AG2-1 and AG3 from Potato(Solanum tuberosum)

作　　者：王宇[1] 杨志辉[1] 秦宇轩[2] 朱杰华[1] 

机构地区：[1]河北农业大学植物保护学院,保定071000 [2]贵州大学生命科学学院,贵阳550025

出　　处：《农业生物技术学报》2013年第2期230-237,共8页Journal of Agricultural Biotechnology

基　　金：现代农业产业技术体系建设专项资金资助(No.CARS-10-P12)

摘　　要：采用菌丝融合分类法对立枯丝核菌进行融合群的划分,有时不能准确地反映该菌群体间的遗传变异。本研究通过菌丝融合反应、ITS序列分析对马铃薯黑痣病菌(Rhizoctonia solani)AG2-1和AG3两个融合群进行了遗传变异研究。对马铃薯黑痣病菌AG2-1和AG3融合反应测定表明,AG3融合群菌株间为完全融合,而AG2-1菌株间存在完全融合和不完全融合现象,根据不完全融合现象可将AG2-1划分为两组。通过ITS序列构建的系统发育树表明,AG3形成单一的分支,而AG2-1可分为明显的两个分支,并且AG2-1和其内部分化出的AG2-1-1和AG2-1-2两个亚分支长度要大于AG3融合群,这说明AG2-1正在发生着明显的遗传分化。基于AG2-1不完全融合反应将其划分的两组与ITS序列分析划分的两个分支完全吻合,因此,不完全融合现象可作为融合群不断分化的参考指标。Current classification within Rhizoctonia solani is largely based on grouping of the population into anastomosis groups （AG）, but does not always appear to accurately reflect the genetic variability among population. Hyphal anastomosis reactions and internal transcribed spacer （ITS） sequences were integrated to evaluate the genetic diversity of isolates Rhizoctonia solani AG2-1 and AG3 from potato （Solarium tuberos urn）. Anastomosis reactions indicated that the complete fusion reaction occurred among the different isolates of AG3. Either the complete fusion reaction or incomplete one occurred among the traditionally defined isolates ofAG2-1. Therefore, AG2-1 was grouped into two sub-groups by incomplete fusion reaction. Phylogenetic tree constructed by ITS sequences showed that the AG3 formed only one separated clade, whereas AG2-1 clustered into the two different sub-clades. The horizontal lengths of the branches indicated that AG2-1 has been appearing obvious genetic differentiation because the relative genetic diversity of AG2-1 or the two subgroup AG2-1-1 and AG2-1-2 were higher than that of AG3. Based on ITS sequences, the two sub-clades within AG2-1 were consistent with the two sub-groups based on the incomplete anastomosis reaction. So the incomplete fusion reaction can be used as a referent character for genetic differentiation within an anastomosis group of Rhizoctonia solani.

关 键 词：立枯丝核菌 菌丝融合 ITS序列 

分 类 号：S532[农业科学—作物学]