电针刺激对2型糖尿病大鼠皮肤屏障及色泽的影响  

Electroacupuncture Intervention Improved Changes of Skin Barrier and Color in Type 2 Diabetes Rats

作　　者：翟煦[1] 荣培晶[1] 王宏才[1] 李少源[1] 孟宏[1] 何孟冬[1] 黄凤[1] 王霞[1] 宋杰[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《针刺研究》2013年第1期7-13,共7页Acupuncture Research

基　　金：科技部"973"课题"经穴效应循经特异性靶器官响应的生物学基础研究"(2012CB 518503);国家科技支撑计划课题"外配式经耳穴迷走神经刺激仪的研发"(2012BAF 14B10)

摘　　要：目的:探讨电针刺激对2型糖尿病大鼠皮肤状态的影响,并比较体穴电针与耳甲区电针作用的差异。方法:50只雄性SD大鼠随机分为空白对照组(n=10)、模型组(n=13)、耳甲区电针刺激组(简称耳甲区组,n=13)和体穴电针刺激组(简称体针组,n=14)。一次性腹腔注射链脲佐菌素,并以高脂饲料喂养30d复制2型糖尿病模型。体针组(取"足三里""三阴交"穴)及耳甲区组大鼠连续接受电针刺激14d。应用皮肤测量系统MPA 9测量造模前、造模后及治疗结束后各组大鼠腹部及背部皮肤的含水量指数(SCH)、水分散失指数(TEWL)、红斑指数(EI)、黑色素指数(MI)、色差值(L.值、a.值、b.值)。结果:造模后,与空白对照组相比,模型组、体针组、耳甲区组背部皮肤SCH、TEWL、EI、MI、L.值、a.值均下降(P<0.05),b.值升高(P<0.05);腹部皮肤的TEWL、EI、a.值、b.值均升高(P<0.05),SCH、L.值均下降(P<0.05),MI没有显著变化(P>0.05)。电针治疗结束后,体针组除腹部TEWL和背部EI变化不明显外(P>0.05),其余指标均较模型组有明显改善(P<0.05);耳甲区组大鼠背部皮肤TEWL、L.值、b.值及腹部皮肤SCH、L.值、b.值较模型组有明显改善(P<0.05),其它指标变化不明显(P>0.05)。结论:电针刺激"足三里""三阴交"或耳甲区后,2型糖尿病大鼠皮肤屏障受损及皮色改变的情况得以改善,体穴电针比耳甲区电针改善作用更明显。Objective To observe the effect of electroacupuncture （EA） of auricular concha region （ACR）, ＂Zusanli＂ （ST 36） and ＂Sanyinjiao＇（SP 6） on abnormal skin barrier and color in streptozotocin （STZ）-induced type 2 diabetes mellitus （T 2 DM） rats so as to find a better therapeutic method. Methods Fifty male Sprague Dawley （SD） rats were randomized into con- trol （n = 10）, model （n = 13）, EA-ST 36-SP 6 （n = 14） and EA-AOR （n = 13） groups. T 2 DM model was established by intra- peritoneal injection of 1% STZ （50 mg/kg） and by feeding the animals with high-fat diet for 30 days. EA （15 Hz, 1 mA） was ap- plied to bilateral ＂Zusanli＂ （ST 36）, ＂Sanyinjiao＂ （SP 6） and AOR respectively for 30 min, once daily for 14 days. Multi Probe Adapter 9 skin measurement system （MPA 9） was used to measure the stratum corneum hydration （SOH）, transepidermal water loss （TEWL）, erythema index （El）, melanin index （MI） and chromatic aberration values （L. a. b. ） of the abdomenal and back （L 1- L6 ） skin, respectively. Results In comparison with the control group and pre-modeling, blood glucose contents of the mo- del, EA-ST 36-SP 6 and EA-AOR groups were increased significantly（P〈0.01） after modeling. Compared with the model group, blood glucose levels of both EA-ST 36-SP 6 and EA-AOR groups were down-regulated apparently （P〈0.01） after treatment. Thirty days after modeling, the SOH, TEWL, El, MI, L. and a. levels of the back skin, and the SOH and L. levels of the ab- dominal skin were decreased significantly （P〈0.05）, while the level of b. in the back skin, and those of TEWL, El, a. and b. of the abdominal skin increased considerably in the model group（P〈0.05）. After EA, the levels of SOH, TEWL MI, L. a. and b. in the EA-ST 36-SP 6 group, SOH, TEWL, L. and b. in the EA-AOR group of the back skin, and SOH, L. and b. of both EA- ST 36-SP 6 and EA-AQR groups, as well as El and a. of the EA-ST 36-SP 6 group of the abdomina

关 键 词：电针 2型糖尿病 链脲佐菌素诱发大鼠 皮肤 耳甲区 

分 类 号：R245.97[医药卫生—针灸推拿学]