长期高果糖饮食喂养对大鼠肝脏脂质沉积和炎症通路的影响  

Effect of high fructose feeding on hepatic steatosis and inflammation pathways in rats

作　　者：任路平[1] 宋光耀[1] 李凡[1] 孙文[1] 胡丽叶[2] 彭兰博[1] 

机构地区：[1]河北省人民医院内分泌一科,河北石家庄050051 [2]解放军白求恩国际和平医院内分泌科

出　　处：《中国老年学杂志》2013年第4期852-854,共3页Chinese Journal of Gerontology

基　　金：河北省科技厅国际合作项目(No.11396406-D)

摘　　要：目的探讨长期(8 w)高果糖饮食对大鼠肝脏脂质沉积及肝脏炎症通路的影响。方法雄性C57BL/J6大鼠分为对照组及高果糖组,经喂养8 w后处死大鼠测定各组肝脏甘油三酯(TG)含量和谷丙转氨酶(ACT)含量,并测定大鼠肝脏内JNK途径(p-JNK/t-JNK)和IκBα途径(p-IKKα/β/t-IKKα/β)的蛋白表达变化。结果喂养8 w后,与对照组相比,高果糖组肝脏甘油三酯和谷丙转氨酶含量较对照组显著增加(P均<0.01);与对照组相比,高果糖组的p-JNK/t-JNK、p-IKKα/β/t-IKKα/β和t-IκB的蛋白表达均显著增加(P均<0.01)。结论 8 w高果糖喂养引起大鼠肝脏脂质沉积,同时伴有JNK和IKKα/β-NF-κB通路激活,提示肝脏两条炎症通路均介导了高果糖喂养诱导脂肪肝和肝胰岛素抵抗的发生发展。Objective To investigate the effect of long-term （8 weeks） high fructose feeding on liver triglyceride （TG） content and hepatic inflammation pathways. Methods Male Wistar rats were fed a standard chow （Con） or high fructose diet （HFru） for 8 weeks. Rats were sacrificed and TG content and alanine transaminase （ALT） in liver were measured. The protein expressions of the markers of two inflammation pathways-JNK pathway （phosphorylated-JNK/total-JNK） and IKKa/β-NF- KB （p-IKKα/β/t-IKKα/β） pathway were detected. Results Compared with control group, TG content and ALT content were significantly increased after high fat feeding （both P 〈 0. 01 ）. The protein expressions of phosphorylated-JNK/total-JNK, phosphorylated -IKKa/β/ total-IKKa/β and IKB were all significantly increased in high-fructose group （P 〈 0. 01 ）. Conclusions 8-week long-term high-fructose feeding can induce liver steatosis in rats. Hepatic steatosis occurres simutaneouly with the activation of both JNK and IKKta/β-NF-KB inflammatory pathways in liver, which indicats both inflammatory pathways are involved in the development of fatty liver induced by high-fructose-feeding.

关 键 词：甘油三酯 脂肪肝 胰岛素抵抗 炎症 

分 类 号：R333.4[医药卫生—人体生理学]