银杏叶提取物对急性肝衰竭大鼠肝脏组蛋白去乙酰化酶2表达的影响  

Effects of Ginkgo biloba extract on liver HDAC2 expression in acute liver failure

作　　者：王树芳[1] 徐海英[2] 李萌[1] 康静[1] 李永海[1] 

机构地区：[1]新乡医学院生命科学技术系,河南新乡453000 [2]黄河科技学院微生物教研室

出　　处：《中国老年学杂志》2013年第4期860-862,共3页Chinese Journal of Gerontology

基　　金：河南省教育厅自然科学研究计划项目(2010A350002);新乡医学院高学历人才资助项目

摘　　要：目的观察银杏叶提取物(GBE)对由硫代乙酰胺(TAA)所致肝损伤的大鼠肝脏的保护作用及其对组蛋白去乙酰化酶2(HDAC2)的影响,并探讨其作用机制。方法实验分5组,正常对照组用生理盐水灌胃和腹腔注射,模型组用生理盐水灌胃,TAA(300 mg/kg)腹腔注射连续3次,间隔24 h,GBE组(50、100、200 mg/kg)用GBE生理盐水灌胃,TAA(300 mg/kg)腹腔注射连续3次,间隔24 h。用试剂盒检测血清中谷草氨酸转移酶(AST)、谷丙氨酸转移酶(ALT)的水平,并用RT-PCR和Western印迹的方法检测各组肝脏HDAC2在mRNA和蛋白水平的表达情况。结果模型组与正常组相比,血清中谷丙氨酸转移酶(ALT)、谷草氨酸转移酶(AST)水平明显升高,肝组织中HDAC2在mRNA和蛋白水平均下调;而GBE组与模型组相比,血清中ALT、AST水平明显降低,肝组织中HDAC2在mRNA和蛋白水平均上调。结论 GBE可通过HDAC2抑制急性肝衰竭大鼠肝细胞凋亡,从而对肝脏起到保护作用。Objective To study the protection effect of ginkgo biloba extract(GBE) on TAA-induced acute liver failure(ALF).Methods 46 rats were divided into five groups: control,model and GBE groups.The model mice of ALF were set up by giving intraperitoneal injection of TAA(350 mg/kg).The serum ALT and AST were determined,HDAC2 was analyzed with RT-PCR.The protein expression of HDAC2 was analyzed with Western blot.Results The serum ALT and AST in mice of model group were increased significantly compared with those of control group.The serum ALT and AST in mice of GBE group were decreased significantly compared with model group.Compared with model group,the gene and protein expressions of HDAC2 were significantly up-regulated in liver of GBE group.Conclusions GBE could protect hepatocytes from apoptosis and necrosis in acute liver injury by TAA in rat and the mechanisms is associated with HDAC2.

关 键 词：急性肝衰竭 硫代乙酰胺 银杏叶提取物 HDAC2 

分 类 号：R285.5[医药卫生—中药学]