响应面法对Acidithiobacillus ferrooxidans发酵液脱除H_2S的优化研究  

Response Surface Optimization for Hydrogen Sulfide Removal Using Fermentation Broth Produced by Acidithiobacillus ferrooxidans

作　　者：晏磊[1] 刘权[1] 张爽[1] 王伟东[1] 王彦杰[1] 荆瑞勇[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《生物技术》2012年第6期86-88,共3页Biotechnology

基　　金：黑龙江省教育厅科学技术研究项目(No.12511361;No.12511362);黑龙江省青年科学基金项目(No.QC2009C115);黑龙江八一农垦大学科研启动基金(No.B2011-13)资助~~

摘　　要：目的:利用Acidithiobacillus ferrooxidans(A.f)发酵液对H2S的脱除条件进行优化。方法:在前期单因素影响脱硫率的基础上,利用Design expert软件中Box-Benhnker中心组合实验设计和响应面分析法对A.f发酵液脱除H2S的气体流量、温度和进行进一步优化。结果:获得了优化的A.f发酵液脱除H2S工艺,反应温度为31.02℃,气体流量为40.03 ml/min,H2S浓度为8.17g/m3。结论:在该条件下脱硫率达99.37%,表明响应面法可有效用于A.f发酵液脱除H2S工艺的优化。Objective：The conditions for hydrogen sulfide removal using fermentation broth produced by AcidithiobaciUus ferrooxidans （A.f） had been optimized. Method： Base on the previous study about the single - factor experiments, Box - Benhnker center composite de- sign from software Design expert and response surface method （RSM） were used for optimization of the gas flow, temperature and hydrogen sulfide concentration. Result：The optimum process parameters for hydrogen sulfide removal using fermentation broth produced by A.f were reaction temperature 31.02℃, gas flow 40. 03 ml/min and hydrogen sulfide concentration 8. 17 g/m3. Conclusion：Under the optimal con- ditions, the removal efficiency of hydrogen sulfide reached 99. 37%, which indicated that the response surface analysis was effective for op- timization of hydrogen sulfide removal using fermentation broth produced by A. f.

关 键 词：硫化氢脱除 响应面法 ACIDITHIOBACILLUS ferrooxidans 优化 

分 类 号：Q89[生物学]