血氧水平依赖MR应用于大鼠Walker-256肿瘤乏氧检测的初步研究  

A preliminary study of murine walker-256 tumor hypoxia detected by blood oxygen level dependent-MR

作　　者：张盛箭[1,2] 毛健[1,2] 吴斌[1,2] 彭卫军[1,2] 

机构地区：[1]复旦大学附属肿瘤医院放射诊断科,上海200032 [2]复旦大学上海医学院肿瘤学系,上海200032

出　　处：《中华放射学杂志》2013年第2期178-182,共5页Chinese Journal of Radiology

基　　金：国家自然科学基金资助项目（30670597）

摘　　要：目的建立大鼠Walker-256移植瘤模型，探索荷瘤大鼠吸人Carbogen气体（95％O2＋5％CO2）后肿瘤R2^＊的信号变化情况及血氧水平依赖（BOLD）-MR技术应用于肿瘤乏氧检测的可能性。方法雌性SD大鼠95只，右下腹皮下接种Walker-256肿瘤细胞。成瘤SD大鼠肿瘤长至1．0～5．0cm时行血氧水平依赖（BOLD）-MR检查。采用美国GE3．0TMR，动物表面线圈及多回波扰相稳态梯度回波（Multi—echo SPGR）序列，于大鼠自由吸入空气时和吸入Carbogen气体10min后分别行BOLD—MR检查。扫描结束后，图像传至GEADW4．3工作站，用R2Star分析软件进行图像后处理，计算肿瘤吸人空气时及吸人Carbogen气体10rain后R2^＊值（R2^＊a、R2^＊b），并求得其变化值△R2^＊=R2^＊ b—R2^＊a，同时计算肿瘤的体积。吸入Carbogen气体前后R2^＊信号差异采用配对t检验，△R2^＊与R2^＊a之间及R2^＊a、△R2^＊与肿瘤体积大小之间行Pearson相关分析。结果接种95只SD大鼠，68只成瘤，成瘤率71．6％。肿瘤体积352～13173mm^3，吸入空气及Carbogen气体后R2^＊值分别为（41．18±22．29）、（38．91±21．35）s^-1，△R2^＊值为（-2．26±3．90）s^-1，差异有统计学意义（t=4．01，P〈0．01）。△R2^＊值与R2^＊值呈负相关（r=-0．32，P〈0．05）。肿瘤的△R2^＊值与肿瘤的体积存在正相关性（r=0．55，P〈0．05），但是肿瘤的R2^＊a值与肿瘤体积之间没有明显相关性（r=-0．03，P〉0．05）。结论BOLD—MRI可以检测SD大鼠吸入Carbogen气体前后Walker-256肿瘤R2^＊信号值的变化。大鼠吸入Carbogen气体后Walker-256肿瘤的R2^＊值会发生明显下降，但不同荷瘤大鼠间差异较大。Objective To establish Walker-256 transplantation tumor model in SD Rats. To study of R2^＊ signal changes on murine Walker-256 tumor after inhaling Carbogen by blood oxygen level dependent （ BOLD ）-MR, and to explore the feasibility of BOLD-MRI on detecting tumor hypoxia. Methods Walker-256 tumor cell implanted subcutaneously in right lower abdomen of 95 female SD rats. MR was performed on the tumor-forming rats when the maximum diameter of tumor reached 1-3 cm, using a 3.0 T MR scanner equipped with a 3 inch animal surface coil. BOLD-MRI was done by using a multi-echo SPGR sequence during inhaling air and at 10 minute after inhaling Carbogen,respectively. All images were transferred to GE ADW 4. 3 workstation,then a baseline R2 ＊ （R2 ＊ a） and R2 ＊ （ R2 ＊ b） after inhaling Carbogen of tumor was calculated using R2Star analysis software and AR2 ＊ was calculated through ＂△R2^＊ =R2^＊ b -R2^＊ a＂, meanwhile the volume of tumor were calculated as well. The difference of R2^＊ signal pre-and post-inhaling of Carbogen was compared with a paired t test, Pearson correlation was calculated between R2^＊ a, △R2^＊ and the volume of tumor, respectively. The correlation between △R2^＊ and R2^＊ a was also assessed by Pearson correlation. Results Sixty-eight of ninety-five female SD rats formed the tumor （71.6%）. The volume of tumor was from 352 to 13 173 mm^3. Mean △R2^＊ decreased significantly （ -2. 26±3.90） s^-1 from （41.18 ±22. 29） s^-1 during breathing air to（38.91 ±21.35 ）s^-1 10 rain after inhaling Carbogen （ t = 4.01 ,P 〈 0.01 ）. △R2^＊ was inversely correlated with R2^＊（ r = - 0. 32, P 〈 0.05 ）. The △R2^＊ was well correlated with volume of tumor （ r = 0. 35, P 〈 0. 05 ） , but R2^＊ a was not correlated with volume of tumor（r = - 0. 03, P 〉 0. 05 ）. Conclusions BOLD-MRI can detect the R2^＊ signal change of murine Walker-256 tumor pre-and post-inhaling of Carbogen. The R2^＊ signal showed significant decrease after

关 键 词：代谢 动物实验 磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学]