碲化钴纳米管/纳米金/壳聚糖复合膜DNA电化学传感器的构建  

Preparation of DNA electrochemical biosensor based on CoTe nanotubes/nano-gold/chitosan composite film

作　　者：孙凯芳[1] 王娇媚[1] 杜丹[1] 范海[1] 艾仕云[1] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018

出　　处：《化学传感器》2012年第4期39-43,共5页Chemical Sensors

摘　　要：通过水热法制备了碲化钴纳米管，以制备的碲化钴纳米管、纳米金和壳聚糖组成的复合膜修饰裸铂电极，构建了一新型DNA电化学生物传感器。利用该生物传感器及邻菲罗啉钴电化学杂交指示剂对禽病毒基因进行了检测研究。实验表明。该法测定DNA的浓度线性范围为2．0x10-10-2．0x10-6mol／L，最低检测限为7．94x10-11mol／L。CoTe nanotubes was synthesis by hydrothermal conditions, and a composite film was mixed with CoTe nanotubes, gold nanoparticles and chitosan. A novel DNA electrochemical biosensor was fabricated with the composite film modified Pt electrode. The avian influenza virus gene sequences was detected using the biosensor and [Co（phen）3]3＋ as electrochemical indicator. The experimental results showed that the linear range for the detection of target DNA is from 2.0×10-10 to 2.0× 10-6 mol/L, and the detection limit is 7.94× 10-11 mol/L.

关 键 词：碲化钴纳米管 纳米金 复合膜 DNA电化学生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]