Bti对三带喙库蚊的杀伤效果及温度的影响  

Killing effect of Bacillus thuringiensis israelensis on Culex tritaeniorhynchus larvae and effect of temperature on the killing

作　　者：马素媛[1,2] 王英[1] 赵清[1] 徐铁龙[3] 吴红渠 艾国平[1] 

机构地区：[1]第三军医大学军事预防医学院热带医学研究所,重庆400038 [2]云南普洱卫生学校,云南普洱6650002 [3]中国疾病预防控制中心寄生虫病所,上海200025 [4]湖北省生物农药工程研究中心,湖北武汉430064

出　　处：《中国热带医学》2012年第12期1427-1429,共3页China Tropical Medicine

基　　金：国家自然科学基金(No.81271875);美国NIH重大课题(No.U19A1089672)

摘　　要：目的对苏云金杆菌以色列亚种(Bacillus thuringiensis israelensis,Bti)杀伤三带喙库蚊幼虫的效果进行生物测定,并探讨温度对Bti杀伤效果的影响。方法首先利用概率分析方法对Bti水分散颗粒剂作用于三带喙库蚊幼虫的杀伤效果进行生物测定,然后检测和比较在不同温度下Bti对三带喙库蚊幼虫的杀伤效果,明确温度对Bti灭蚊效果的影响。结果 Bti对三带喙库蚊幼虫的灭杀起效快,12h内即显示很强的杀伤效果。LC50和LC95分别是171.72 IU/L和646.60 IU/L。24h内温度(16-37℃)对杀伤效果有一定的影响,适当提高环境温度,可以增强Bti对蚊幼虫的杀伤效果。结论三带喙库蚊幼虫对Bti较为敏感,一定范围内升高温度可以提高Bti的起效速度和杀伤效果。本研究结果提示,Bti是三带喙库蚊防制的理想生物杀虫剂。Objective To study the Killing effect of Bacillus thuringiensis israelensis （Bfi） on Culex tritaeniorhynchus larvae and the effect of temperature on the killing. Methods Firstly, bioassay was carried out to test the mortality of Culex tritaeniorhynchus larvae after Bti treatment by probit analysis and the killing effect of Bti on C tfftaeniorhynchus under different temperature was investigated. Results C. tfftaeniorhynchus larvae were quickly killed by Bti 12 hours after treatment with Bfi LCs0 and LC95 were 171.72 IU/L and 646.60 IU/L respectively. And, the killing etficiency was significantly affected by temperature within the range of 16~37~C in 24 hours. But there was no significant difference between groups 12h and 24h post treatment at 28~C and 37~C. Conclusion Culex tritaeniorhynchus larvae are sensitive to Bti. The Killing Effect of Bti on C tfitaeniorhynchus larvae could be accelerated and improved by enhancemnent of the emperature in a limited range. The results show that Bti is a kind of ideal larvicide to control C tritaeniorhynchus.

关 键 词：苏云金杆菌以色列亚种 三带喙库蚊 生物测定 温度 

分 类 号：R811.5[医药卫生—放射医学]