新疆维吾尔族HIV-1感染者gag基因序列分型研究  

Study on subtypes of gag gene of HIV-infected patients in Xinjiang Uygur Autonomous Region

作　　者：米吉提.买买提 周素娟[2] 王发省[1] 常燕玲 艾尼 芮宝玲[5] 王丽[1] 王昌敏[1] 高眉扬[2] 何纳[2] 

机构地区：[1]新疆维吾尔自治区人民医院预防保健科,新疆乌鲁木齐830001 [2]复旦大学公共卫生学院流行病学教研室,上海200032 [3]伊宁市人民医院,新疆伊宁835000 [4]乌鲁木齐市水磨沟区人民医院,新疆乌鲁木齐830002 [5]乌鲁木齐市疾病预防控制中心,新疆乌鲁木齐830001

出　　处：《中国热带医学》2012年第12期1430-1432,1456,共4页China Tropical Medicine

基　　金：新疆维吾尔自治区科技支疆项目计划资助(No.201191248)

摘　　要：目的通过扩增新疆地区HIV-1感染者gag基因片段,确定HIV-1感染者流行株的基因亚型。方法抽提88例新疆乌鲁木齐市和伊宁市HIV-1感染者的血浆标本的RNA,用巢式PCR扩增gag基因片段,将所得到的序列与国际标准株进行比较,确定被检标本的亚型。结果在88例HIV感染者的血浆标本中,共成功扩增57例gag基因片段,扩增率为64.8%。经BLAST初步判断和构建进化树并与国际标准株比对确认,57例感染者感染的HIV毒株序列中,有56个为CRF07_BC亚型,1例为C亚型。57例毒株之间的平均基因距离为0.043±0.003,与3例CRF07_BC亚型国际参考株之间的平均基因距离在0.034±0.004-0.039±0.005之间。结论新疆地区HIV-1感染者以CRF07_BC亚型为主要流行株。57例序列相互之间以及与3株不同来源的国际参考毒株之间表现出高度的同源性。Objective To explore the prevalent subtype of gag gene from HIV-1 infected patients in Xinjiang. Meth- ods The HIV-1 RNA was extracted from HIV-1 positive plasma from 88 HIV-1 infected patients in Xinjiang, then HIV-1 gag gene were amplified by nest-PCR, and was further subject to the DNA sequence and subtype analysis. Results The gag gene of 57 samples out of the 88 HIV-1 patients were successfully amplified by PCR accounted for 64.8%. Two HIV-1 sub- types, CRF07_BC（56 strains）,C（1 strain） were identified. Average gene distance of the 57 sequence was 0.043±0.003.The aver- age gene distance to the 3 international standard strains were between 0.034±0.004 and 0.039±0.005. Conclusions The prevalent subtype of HIV-1 infected patients in Xinjiang was CRF07_BC. All the 57 sequences and 3 international standard strains were highly homogenous.

关 键 词：HIV感染者 GAG 基因分型 巢式PCR 

分 类 号：R512.91[医药卫生—内科学]