脑尔康对AD模型大鼠海马内低密度脂蛋白受体相关蛋白表达的影响  

Effects of NaoerKang on the Expression of LDL Receptor-related Protein in Hippocampus in the Rat Model with Alzheimer's Disease

作　　者：袁海峰[1] 李玺[1] 权乾坤[1] 王宁宁[1] 刘颖[1] 李明[1] 

机构地区：[1]西安交通大学医学院第二附属医院,西安710004

出　　处：《河北北方学院学报（自然科学版）》2012年第5期59-63,68,共6页Journal of Hebei North University:Natural Science Edition

基　　金：陕西省科技攻关项目[编号:2007K16-07(5)]

摘　　要：目的观察复方中药脑尔康对阿尔茨海默病(AD)模型大鼠海马低密度脂蛋白受体相关蛋白(LDL receptor-related protein,LRP)表达的影响,探讨其抗痴呆的作用机制。方法 48只健康雄性SD大鼠随机分为空白组、模型组、吡拉西坦组和脑尔康大、中、小剂量组,每组8只。除空白组外,其余各组大鼠双侧海马CA1区各一次性注射10μg凝聚态Aβ1-42制备AD模型,空白组注射等体积生理盐水。3个脑尔康剂量组大鼠给予脑尔康(60、30、15g.kg-1.d-1)连续灌胃28d,吡拉西坦组给予吡拉西坦(0.375g.kg-1.d-1)灌胃,空白组和模型组给予等量生理盐水灌胃。采用Y型电迷宫检测大鼠学习记忆能力,采用免疫组化染色法检测海马内LRP表达。结果大鼠海马注射Aβ1-42后,与空白组比较,模型组大鼠学习记忆能力下降,海马内Aβ1-42表达明显增多,而LRP的表达减少;经脑尔康干预后,与模型组比较,吡拉西坦组和脑尔康组大鼠学习记忆能力改善(P<0.05或P<0.01),海马内Aβ1-42表达下降(P<0.05或P<0.01),而LRP的表达上调(P<0.05或P<0.01)。结论复方中药脑尔康可能通过上调AD模型大鼠海马LRP的表达从而降低Aβ1-42的含量,并改善其学习记忆能力,从而发挥抗痴呆作用。Objectives To investigate the effects of NaoerKang(NEK),a compound traditional Chinese herbal drug,on the expression of LDL receptor-related protein(LRP)in hippocampus of the rat model with Alzheimer' disease(AD).Methods Forty-eight healthy male SD rats were randomly divided into control,untreated and piracetam groups,as well as low-dose,medium-dose and high-dose NEK groups,with 8 in each group.10 μg of Aβ1-42 was injected into both CA1 area of hippocampus to prepare rat model of AD,whereas the control rats were injected with same volume of normal saline.The rats in NEK groups were treated respectively with high-,medium-and low-dose[60,30,15,g·kg-1·d-1] of NEK for 28 days consecutively;piracetam[0.375 g·kg-1·d-1]was intragastrically administered to rats in the piracetam group;and normal saline was applied in control and model groups.Y-maze test was performed to test the learning and memory abilities of rats in different groups.Immunohistochemistry staining was used to determine the expression of LRP in hippocampus,and results were analyzed by Image Acquisition and Analysis System.Results Injection of Aβ1-42 induced learning and memory dysfunction and up-regulated the expression of Aβ1-42 in hippocampus in the model group.Compared with that of normal control group,learning and memory abilities of rats in the model group were decreased(P<0.01) and the expression of Aβ1-42 was significantly increased(P<0.01) after Aβ1-42 injection.After 28-day administration of NEK and piracetam,the learning and memory abilities of rats in piracetam and NEK groups were significantly improved,(P<0.01 or P<0.05) and the expression of Aβ1-42 was decreased(P<0.01 or P<0.05) and the expression of LRP was increased(P<0.01or P<0.05),compared with that of model group.Conclusion NEK could reduce Aβ1-42 level in hippocampus of the rat model of AD by increasing the expression of LRP,and as a result,improve the learning and memory abilities of rats.

关 键 词：脑尔康 阿尔茨海默病 低密度脂蛋白受体相关蛋白 

分 类 号：R969[医药卫生—药理学]