电子目镜显微镜的景深研究  

Study on the Depth of Field for Microscope Equipped with an Electronic Eyepiece

作　　者：孙丽存[1] 李宇[1] 孟伟东[1] 李强[1] 普小云[1] 

机构地区：[1]云南大学物理科学技术学院物理系,云南昆明650091

出　　处：《光学学报》2012年第F12期111-118,共8页Acta Optica Sinica

基　　金：国家自然科学基金（11164033）、云南省应用基础研究基金（2011FA006）和云南省高校科技创新团队支持计划资助课题.

摘　　要：基于近轴条件下的高斯公式，导出了电子目镜显微镜的几何和物理景深公式并分析了其适用的条件。通常情况下，电子目镜显微镜的景深值由其物理景深决定，景深值与显微物镜的有效数值孑L径的平方成反比，与电子目镜的放大倍数无直接关系。用Zemax软件模拟了10×20电子目镜显微镜的景深，软件模拟值与计算值完全相符。为了验证景深公式，设计了毛细管测量系统，用其测量了有效数值孔径不同时电子目镜显微镜的景深以及有效数值孔径等于显微物镜数值孔径时不同规格电子目镜显微镜的景深。实验测量值与计算值十分吻合。Based on the Gaussian formula under the condition of paraxial approximation, formula determining the geometric depth of field and physical depth of field for the microscope equipped with an electronic eyepiece are deduced, and the application conditions are analyzed. Usually, the depth of field for imaging system is depend on its diffraction limit, then the depth of field for the microscope is inversely proportional to the square of the effective numerical aperture, and has no direct relationship with the magnification of the electronic eyepiece. An optical simulation for a microscope （the transversal magnification is 10 X 20） is carried out by using Zemax software, the obtained result is consistent with the deduced formula. To verify the deduced formula, a capillary measurement system is designed. The depth of field for microscope is then measured experimentally by varying its effective numerical aperture and the depths of field for different types of microscopes are measured. The measured depths of field are in good consistent with the data calculated by the deduced formula.

关 键 词：成像系统 景深 电子目镜 有效数值孔径 

分 类 号：O439[机械工程—光学工程]