中心复合设计-效应面法优化玄参的炮制工艺  

Optimization of Processing Technology for Scrophularia ningpoensis by Central Composite Design-Response Surface Methodology

作　　者：周文杰[1,2] 傅亚[3] 王云红[1] 张小梅[1] 柯秀梅[1,2] 刘楠[1,2] 杨荣平[1,2] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]成都中医药大学,成都611137 [3]重庆科技学院化学化工学院,重庆401331

出　　处：《中国实验方剂学杂志》2013年第4期10-14,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI13B03)

摘　　要：目的:优选玄参的炮制工艺条件。方法:采用中心复合设计-效应面法,以浸泡时间、蒸制时间、干燥温度为考察因素,哈帕苷、哈帕俄苷、肉桂酸变化率的总评归一值(OD)为指标,进行中心复合设计试验,并用多元线性回归及二项式拟合建立指标与因素间的数学模型,预测玄参的最佳炮制工艺。结果:最佳工艺为浸泡19 min,蒸制1.2 h,干燥温度49℃。预测值与实测值偏差均<2.65%。结论:采用中心复合设计-效应面法优化的玄参炮制工艺稳定可行,结果可靠,为玄参的质量标准研究提供实验依据。Objective： To optimize processing technology conditions of Scrophularia ningpoensis. Method： Central composite design and response surface method was used with soaking time, steaming time, drying temperature as factors, OD of change ratio of harpagide, cinnamic acid and harpagoside as index, mathematical model of index and factors were established by multi-linear regression and binomial fitting, and to predict optimum processing technology of S. ningpoensis. Result： Optimal conditions were as followings： soaking time 19 min, steaming time 1.2 h, drying temperature 49 ℃. Deviation of predicted and measured value was less than 2.65%. Conclusion： Optimized processing technology was stable, reliable and feasible, it could provide experimental evidence for quality standards research of S. ningpoensis.

关 键 词：玄参 炮制工艺 中心复合设计 效应面法 

分 类 号：R283.6[医药卫生—中药学]